Hsp25/Hsp27 Antibody
Product Sizes
100ug
£511.00
OASE00024-100UG
About this Product
- SKU:
- OASE00024
- Additional Names:
- HSPB1; Heat shock protein beta-1; HSPB1; DMPK-binding protein; MKBP
- translate.label.attr.clone:
- 8A7
- Clonality:
- Monoclonal
- Conjugate:
- Unconjugated
- Concentration:
- 1 mg/ml
- Extra Details:
- Hsp25 is the mouse homologue of the human Hsp27 protein; a member of the small heat shock protein family comprised of a diverse group of proteins from ~15 to >30kDa(1). The basic structure of most sHsps is a homologous and highly conserved amino acid sequence; with an alpha-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (2; 3). Hsp27-oligomers consist of stable dimers formed by as many as 8-40 Hsp27 protein monomers (4). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity; whereas dimers have no chaperone activity (5). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress; where it may function to stabilize DNA and/or the nuclear membrane. It can be rapidly phosphorylated in response to physiological stimuli relevant to the cell type examined. Thus; hsp27 has been suggested to be an important intermediate in second messenger-mediated signaling pathways (6). Other functions include chaperone activity (as mentioned above); thermo-tolerance in vivo; inhibition of apoptosis; and signal transduction.
Specifically; in vitro; it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins; which ensures refolding of the HSP70 complex. Hsp27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex; thereby inhibiting the activation of procaspase-9. It is also hypothesized that hsp27 may serve some role in cross-bridge formation between actin and myosin (7). And finally; Hsp27 is also thought to be involved in the process of cell differentiation. The up-regulation of Hsp27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that Hsp27 may play a crucial role in termination of growth (8). - Gene Details:
- heat shock 27kDa protein 1
- Host:
- Mouse
- Immunogen:
- HSP27 peptide
- Isotype:
- IgG1 Kappa
- Molecular Weight:
- 25 or 27 kD
- Purification:
- Protein G Purified
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- Store at -20C. Shipping with Blue Ice or 4C.
- Supplier:
- Aviva Systems Biology
- Type:
- Antibodies: Monoclonal Antibody
- Manufacturer's Data Sheet:html_datasheet.php
