ACE2 Antibody
Product Sizes
ACE2 Antibody
£ POA
OAPB00214
About this Product
- SKU:
- OAPB00214
- Application:
- ELISA, Western Blot, IHC-Paraffin, Immunofluorescence
- Clonality:
- Polyclonal
- Concentration:
- 1 mg/mL
- Format:
- Liquid. Supplied in PBS containing 0.02% sodium azide.
- Gene Details:
- ACE2
- Host:
- Rabbit
- Isotype:
- IgG
- Molecular Weight:
- Predicted: 93kDa<br/>Observed: 130 kDa (7 N-linked glycosylation)
- Protein Details:
- Angiotensin-converting enzyme 2
- Purification:
- ACE2 Antibody is affinity chromatography purified via peptide column.
- References:
- Donoghue et al. Circ. Res. 2000;87:1-9.<br/>Tipnis et al. J Biol. Chem. 2000;275:33238-43.<br/>Li et al. Nature 2003;426:450-4.<br/>Lu et al. The Lancet 2020 (published online).<br/>Crackower et al. Nature 2002;417:822-8.
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- Stored at 4C for three months and -20C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- Supplier:
- Aviva Systems Biology
- Manufacturer's Data Sheet:html_datasheet.php
Loading: 15 ug of lysates per lane. Antibodies: OAPB00213 (2 ug/mL), OAPB00214 (2 ug/mL), OAPB00219 (2 ug/mL) and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Loading: 15 ug of lysates per lane. Antibodies: ACE2 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Loading: 15 ug of lysates per lane. Antibodies: ACE2 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Loading: 15 ug of lysates per lane. Antibodies: ACE2 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ACE2 antibody at 2 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.