Mouse Anti-FLAG Antibody
Product Sizes
0.5mg
£485.00
OAPA00047-0.5MG
About this Product
- SKU:
- OAPA00047
- translate.label.attr.clone:
- NYRFLAG
- Clonality:
- Monoclonal
- Extra Details:
- A fusion tag called FLAG consists of eight amino acids (AspTyrLysAspAspAspAspLys) including an enterokinase-cleavage site. FLAG is specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody. denoted as M1. binds the peptide in the presence of bivalent metal cations. preferably Ca(+). Elution is effected by chelating agents. Another strategy is competitive elution with excess of free Flag Peptide. The FLAG peptide purifies and detects recombinant fusion proteins. FLAG peptide is useful in Western blotting; immunocytochemistry; immunoprecipitation; flow cytometry; protein purification; and in the study of protein-protein interactions; cell ultrastructure; and protein localization. Flag peptide is a hydrophilic tag which significantly improves the detection and purification of recombinant fusion proteins.
- Host:
- Mouse
- Immunogen:
- Flag-conjugated proteins.
- Isotype:
- IgG1; kappa.
- Purification:
- Protein A & Ion exchange column.
- Shipping Conditions:
- Ambient
- Source:
- BALB/c mice
- Storage Conditions:
- Reconstitute with H20 to get a 1 mg/ml concentration. Mix gently; wash the sides of the vial and wait 30-60 seconds before use. In lyophilized form; for long periods; store at 4C in a dry environment. After reconstitution; if not intended for use within a month; aliquot and store at -20C.
- Supplier:
- Aviva Systems Biology
- Type:
- Antibodies: Monoclonal Antibody
- Manufacturer's Data Sheet:html_datasheet.php
