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Antibodies

5-hmC Antibody

Product Sizes
5-hmC Antibody
£ POA
OAGA08408
Academic Pricing
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About this Product
SKU:
OAGA08408
Application:
Dot Blot, Immunoprecipitation
Clone:
GT513
Clonality:
Monoclonal
Concentration:
1 mg/ml
Format:
Liquid
Gene Details:
5-hmC
Host:
Mouse
Isotype:
IgG2b
Purification:
Affinity purified by Protein G.
Shipping Conditions:
Blue Ice
Storage Conditions:
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Supplier:
Aviva Systems Biology
5-Hydroxymethylcytosine/ 5-hmC antibody [GT513] in MeDIP experiment.
Human genomic DNA (500 ng) mixed with methylated cytosine standard kit were subjected to MeDIP with 5-Hydroxymethylcytosine/ 5-hmC antibody [GT513] at dilution of 1:500 and analyzed with semi-quantitative PCR. The immunoprecipitated DNA was plotted as % of input DNA.
5-hydroxymethylcytosine antibody [GT513] Dot blot analysis of anti-5-hmC antibody with the synthetic DNA controls . DNA samples (0.5 to 50 ng as indicated) were spotted onto the positively charged Nylon membrane and blotted with 5-hmC antibody at a dilution of 1:500. A: Unmethylated DNA fragment B: DNA fragment containing 5-methylcytosine C: DNA fragment containing 5-hydroxymethylcytosine D: DNA fragment containing 5-formylcytosine E: DNA fragment containing 5-carboxylcytosine
Dotblot analysis of anti- 5-Hydroxymethylcytosine / 5-hmC antibody [GT513] with DNA samples
DNA samples (50 to 500 ng as indicated) were spotted onto positively charged nylon membrane and blotted with 5-Hydroxymethylcytosine / 5-hmC antibody [GT513] at a dilution of 1:500.
A: Genomic DNA derived from 293T cells.
B: Genomic DNA derived from human TET-1 transfected 293T cells.
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