6X His tag Antibody
Product Sizes
6X His tag Antibody
£ POA
OAGA08349
About this Product
- SKU:
- OAGA08349
- Application:
- Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot, ELISA
- Clone:
- GT359
- Clonality:
- Monoclonal
- Concentration:
- 1 mg/ml
- Format:
- <b>Liquid</b> PBS
- Gene Details:
- 6X His tag
- Host:
- Mouse
- Isotype:
- IgG3
- Purification:
- Affinity purified by Protein G.
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- Keep as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
- Supplier:
- Aviva Systems Biology
- Manufacturer's Data Sheet:html_datasheet.php
Lane A and B were IPTG-induced cell lysate from E. coli expressing different GST-tagged proteins.
Lane C to G were IPTG induced cell lysate from E. coli expressing different His-tagged protein.
12% SDS-PAGE
6X His tag antibody [GT359] dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
A. Non-induced cell lysate from E. coli expressing His-tagged proteins
B. IPTG-induced cell lysate from E. coli expressing His-tagged proteins
12% SDS-PAGE
6X His tag antibody [GT359] dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
A. 2 ug GST-recombinant protein
B. 2 ug His-recombinant protein
C. 1 ug His-recombinant protein
D. 0.5 ug His-recombinant protein
E. 0.1 ug His-recombinant protein
F. 0.05 ug His-recombinant protein
12% SDS-PAGE
6X His tag antibody [GT359] dilution: 1:3000
The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
IP Sample: cell lysate/extract of His-fused gene transfected HeLa cells
A. 1/20 lysate/extract of transfected HeLa cells
B. Control with 2 ug of preimmune mouse IgG
C. Immunoprecipitation of His-fused protein by 2 ug of 6X His tag antibody [GT359]
12% SDS-PAGE
The immunoprecipitated His-fused protein was detected by 6X His tag antibody [GT359] diluted at 1:1000.