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Antibodies

5-hmC Antibody

Product Sizes
5-hmC Antibody
£ POA
OAGA07328
Academic Pricing
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About this Product
SKU:
OAGA07328
Application:
Dot Blot, Immunoprecipitation
Clonality:
Polyclonal
Concentration:
7.55 mg/ml
Format:
Liquid
Gene Details:
5-hmC
Host:
Rabbit
Isotype:
IgG
Purification:
Purified by antigen-affinity chromatography.
Shipping Conditions:
Blue Ice
Storage Conditions:
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Supplier:
Aviva Systems Biology
5-Hydroxymethylcytosine/ 5-hmC antibody in MeDIP experiment.
Human genomic DNA (150 ng) mixed with methylated cytosine standard kit were subjected to MeDIP with 5-Hydroxymethylcytosine/ 5-hmC antibody at dilution of 1:2000 and analyzed with semi-quantitative PCR. The immunoprecipitated DNA was plotted as % of input DNA.
5-hydroxymethylcytosine antibody Dot blot analysis of anti-5-hmC antibody with the synthetic DNA controls . DNA samples (0.5 to 50 ng as indicated) were spotted onto the positively charged Nylon membrane and blotted with 5-hmC antibody at a dilution of 1:50000.
A: Unmethylated DNA
B: DNA containing 5-methylcytosine
C: DNA containing 5-hydroxymethylcytosine
D: DNA containing 5-formylcytosine
E: DNA containing 5-carboxylcytosine
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