UPF1 Antibody
Product Sizes
50ug
£305.00
OACA10232-50UG
100ug
£463.00
OACA10232-100UG
About this Product
- SKU:
- OACA10232
- Additional Names:
- ATP-dependent helicase RENT1;delta helicase;HUPF1;nonsense mRNA reducing factor 1;NORF1;pNORF1;regulator of nonsense transcripts 1;RENT1;smg-2;smg-2 homolog; nonsense mediated mRNA decay factor;UPF1 regulator of nonsense transcripts homolog;up-frameshift mutation 1 homolog;up-frameshift suppressor 1 homolog;UTF;yeast Upf1p homolog.
- Clonality:
- Polyclonal
- Conjugate:
- Unconjugated
- Extra Details:
- RNA-dependent helicase and ATPase required for nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited to mRNAs upon translation termination and undergoes a cycle of phosphorylation and dephosphorylation; its phosphorylation appears to be a key step in NMD. Recruited by release factors to stalled ribosomes together with the SMG1C protein kinase complex to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD; the SURF complex associates with the exon junction complex (EJC) (located 50-55 or more nucleotides downstream from the termination codon) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Phosphorylated UPF1 is recognized by EST1B/SMG5; SMG6 and SMG7 which are thought to provide a link to the mRNA degradation machinery involving exonucleolytic and endonucleolytic pathways; and to serve as adapters to protein phosphatase 2A (PP2A); thereby triggering UPF1 dephosphorylation and allowing the recycling of NMD factors. UPF1 can also activate NMD without UPF2 or UPF3; and in the absence of the NMD-enhancing downstream EJC indicative for alternative NMD pathways. Plays a role in replication-dependent histone mRNA degradation at the end of phase S; the function is independent of UPF2. For the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed. The ATPase activity of UPF1 is required for disassembly of mRNPs undergoing NMD. Essential for embryonic viability.
- Gene Details:
- UPF1 RNA helicase and ATPase
- Host:
- Rabbit
- Immunogen:
- Recombinant Human Regulator of nonsense transcripts 1 protein (956-1125AA)
- Isotype:
- IgG
- Protein Details:
- Regulator of nonsense transcripts 1
- Purity:
- >95%
- Purification:
- Protein G Purified
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- -20Â[o]C or -80Â[o]C
- Supplier:
- Aviva Systems Biology
- Type:
- Antibodies: Polyclonal Antibody
- Manufacturer's Data Sheet:html_datasheet.php
