HLA-DQA1 Antibody - HRP Conjugated
Product Sizes
100ug
£478.00
OACA02036-100UG
About this Product
- SKU:
- OACA02036
- Additional Names:
- CELIAC1;DC-1 alpha chain;DC-alpha;DQA1;DQ-A1;HLA class II histocompatibility antigen DQ alpha chain;HLA class II histocompatibility antigen; DQ alpha 1 chain;HLA-DCA;HLA-DQA;MHC class II antigen DQA1;MHC class II DQ alpha chain;MHC class II DQA1;MHC class II HLA-DQ-alpha-1;MHC class II protein;MHC HLA-DQ alpha;truncated MHC class II antigen.
- Clonality:
- Polyclonal
- Conjugate:
- HRP
- Extra Details:
- Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route; where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules; and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments; exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides; autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs; other cells of the gastrointestinal tract; such as epithelial cells; express MHC class II molecules and CD74 and act as APCs; which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen; three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs; CD74 undergoes a sequential degradation by various proteases; including CTSS and CTSL; leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells; the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules; increased acidification produces increased proteolysis and efficient peptide loading.
- Gene Details:
- major histocompatibility complex; class II; DQ alpha 1
- Host:
- Rabbit
- Immunogen:
- Recombinant Human HLA class II histocompatibility antigen; DQ alpha 1 chain protein (24-213AA)
- Isotype:
- IgG
- Protein Details:
- HLA class II histocompatibility antigen; DQ alpha 1 chain
- Purity:
- Caprylic Acid Ammonium Sulfate Precipitation purified
- Purification:
- Protein G Purified
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- -20Â[o]C or -80Â[o]C
- Supplier:
- Aviva Systems Biology
- Type:
- Antibodies: Polyclonal Antibody
- Manufacturer's Data Sheet:html_datasheet.php
