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Antibodies

IL27 Antibody

Product Sizes
100 uL
£446.00
OAAJ07843-100UL
About this Product
SKU:
OAAJ07843
Additional Names:
p28; IL30; IL-27; IL27A; IL-27A; IL27p28
Clonality:
Polyclonal
Concentration:
1 mg/mL
Extra Details:
Associates with EBI3 to form the IL-27 interleukin; a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties; that can regulate T-helper cell development; suppress T-cell proliferation; stimulate cytotoxic T-cell activity; induce isotype switching in B-cells; and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1); type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells; binds to the cytokine receptor WSX-1/TCCR which appears to be required but not sufficient for IL-27-mediated signal transduction. IL-27 potentiate the early phase of TH1 response and suppress TH2 and TH17 differentiation. It induces the differentiation of TH1 cells via two distinct pathways; p38 MAPK/TBX21- and ICAM1/ITGAL/ERK-dependent pathways. It also induces STAT1; STAT3; STAT4 and STAT5 phosphorylation and activates TBX21/T-Bet via STAT1 with resulting IL12RB2 up-regulation; an event crucial to TH1 cell commitment. It suppresses the expression of GATA3; the inhibitor TH1 cells development. In CD8 T-cells; it activates STATs as well as GZMB. IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production. Indeed IL27 alone is also able to inhibit the production of IL17 by CD4 and CD8 T-cells. While IL-27 suppressed the development of proinflammatory Th17 cells via STAT1; it inhibits the development of anti-inflammatory inducible regulatory T-cells; iTreg; independently of STAT1. IL-27 has also an effect on cytokine production; it suppresses proinflammatory cytokine production such as IL2; IL4; IL5 and IL6 and activates suppressors of cytokine signaling such as SOCS1 and SOCS3. Apart from suppression of cytokine production; IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T-cells. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines such as IP-10/CXCL10 and MIG/CXCL9. In vein endothelial cells; it induces IRF1/interferon regulatory factor 1 and increase the expression of MHC class II transactivator/CIITA with resulting up-regulation of major histocompatibility complex class II. IL-27 also demonstrates antiviral activity with inhibitory properties on HIV-1 replication.
Host:
Rabbit
Immunogen:
Recombinant protein of human IL27
Molecular Weight:
27 kDa
Protein Details:
Interleukin-27 subunit alpha
Purification:
Immunogen affinity chromatography
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4C. For long term storage; aliquot and store at -20C. Avoid freeze/thaw cycles.
Supplier:
Aviva Systems Biology
Type:
Antibodies: Polyclonal Antibody