BMAL1 Antibody
Product Sizes
100 ug
£496.00
OAAF08218-100UG
About this Product
- SKU:
- OAAF08218
- Additional Names:
- ARNT-like protein 1; brain and muscle;aryl hydrocarbon receptor nuclear translocator-like protein 1;basic helix-loop-helix family member e5;basic-helix-loop-helix-PAS orphan MOP3;basic-helix-loop-helix-PAS protein MOP3;bHLHe5;bHLH-PAS protein JAP3;BMAL1;BMAL1c;brain and muscle ARNT-like 1;class E basic helix-loop-helix protein 5;JAP3;member of PAS protein 3;member of PAS superfamily 3;MOP3;PAS domain containing 3;PAS domain-containing protein 3;PASD3;testis tissue sperm-binding protein Li 50e;TIC.
- Clonality:
- Polyclonal
- Concentration:
- 1 mg/ml
- Extra Details:
- Transcriptional activator which forms a core component of the circadian clock. The circadian clock; an internal time-keeping system; regulates various physiological processes through the generation of approximately 24 hour circadian rhythms in gene expression; which are translated into rhythms in metabolism and behavior. It is derived from the Latin roots 'circa' (about) and 'diem' (day) and acts as an important regulator of a wide array of physiological functions including metabolism; sleep; body temperature; blood pressure; endocrine; immune; cardiovascular; and renal function. Consists of two major components: the central clock; residing in the suprachiasmatic nucleus (SCN) of the brain; and the peripheral clocks that are present in nearly every tissue and organ system. Both the central and peripheral clocks can be reset by environmental cues; also known as Zeitgebers (German for 'timegivers'). The predominant Zeitgeber for the central clock is light; which is sensed by retina and signals directly to the SCN. The central clock entrains the peripheral clocks through neuronal and hormonal signals; body temperature and feeding-related cues; aligning all clocks with the external light/dark cycle. Circadian rhythms allow an organism to achieve temporal homeostasis with its environment at the molecular level by regulating gene expression to create a peak of protein expression once every 24 hours to control when a particular physiological process is most active with respect to the solar day. Transcription and translation of core clock components (CLOCK; NPAS2; ARNTL/BMAL1; ARNTL2/BMAL2; PER1; PER2; PER3; CRY1 and CRY2) plays a critical role in rhythm generation; whereas delays imposed by post-translational modifications (PTMs) are important for determining the period (tau) of the rhythms (tau refers to the period of a rhythm and is the length; in time; of one complete cycle). A diurnal rhythm is synchronized with the day/night cycle; while the ultradian and infradian rhythms have a period shorter and longer than 24 hours; respectively. Disruptions in the circadian rhythms contribute to the pathology of cardiovascular diseases; cancer; metabolic syndromes and aging. A transcription/translation feedback loop (TTFL) forms the core of the molecular circadian clock mechanism. Transcription factors; CLOCK or NPAS2 and ARNTL/BMAL1 or ARNTL2/BMAL2; form the positive limb of the feedback loop; act in the form of a heterodimer and activate the transcription of core clock genes and clock-controlled genes (involved in key metabolic processes); harboring E-box elements (5'-CACGTG-3') within their promoters. The core clock genes: PER1/2/3 and CRY1/2 which are transcriptional repressors form the negative limb of the feedback loop and interact with the CLOCK|NPAS2-ARNTL/BMAL1|ARNTL2/BMAL2 heterodimer inhibiting its activity and thereby negatively regulating their own expression. This heterodimer also activates nuclear receptors NR1D1/2 and RORA/B/G; which form a second feedback loop and which activate and repress ARNTL/BMAL1 transcription; respectively. ARNTL/BMAL1 positively regulates myogenesis and negatively regulates adipogenesis via the transcriptional control of the genes of the canonical Wnt signaling pathway. Plays a role in normal pancreatic beta-cell function; regulates glucose-stimulated insulin secretion via the regulation of antioxidant genes NFE2L2/NRF2 and its targets SESN2; PRDX3; CCLC and CCLM. Negatively regulates the mTORC1 signaling pathway; regulates the expression of MTOR and DEPTOR. Controls diurnal oscillations of Ly6C inflammatory monocytes; rhythmic recruitment of the PRC2 complex imparts diurnal variation to chemokine expression that is necessary to sustain Ly6C monocyte rhythms. Regulates the expression of HSD3B2; STAR; PTGS2; CYP11A1; CYP19A1 and LHCGR in the ovary and also the genes involved in hair growth. Plays an important role in adult hippocampal neurogenesis by regulating the timely entry of neural stem/progenitor cells (NSPCs) into the cell cycle and the number of cell divisions that take place prior to cell-cycle exit. Regulates the circadian expression of CIART and KLF11. The CLOCK-ARNTL/BMAL1 heterodimer regulates the circadian expression of SERPINE1/PAI1; VWF; B3; CCRN4L/NOC; NAMPT; DBP; MYOD1; PPARGC1A; PPARGC1B; SIRT1; GYS2; F7; NGFR; GNRHR; BHLHE40/DEC1; ATF4; MTA1; KLF10 and also genes implicated in glucose and lipid metabolism. Promotes rhythmic chromatin opening; regulating the DNA accessibility of other transcription factors. The NPAS2-ARNTL/BMAL1 heterodimer positively regulates the expression of MAOA; F7 and LDHA and modulates the circadian rhythm of daytime contrast sensitivity by regulating the rhythmic expression of adenylate cyclase type 1 (ADCY1) in the retina. The preferred binding motif for the CLOCK-ARNTL/BMAL1 heterodimer is 5'-CACGTGA-3'; which contains a flanking Ala residue in addition to the canonical 6-nucleotide E-box sequence (PubMed:23229515). CLOCK specifically binds to the half-site 5'-CAC-3'; while ARNTL binds to the half-site 5'-GTGA-3' (PubMed:23229515). The CLOCK-ARNTL/BMAL1 heterodimer also recognizes the non-canonical E-box motifs 5'-AACGTGA-3' and 5'-CATGTGA-3' (PubMed:23229515). Essential for the rhythmic interaction of CLOCK with ASS1 and plays a critical role in positively regulating CLOCK-mediated acetylation of ASS1 (PubMed:28985504).
- Gene Details:
- aryl hydrocarbon receptor nuclear translocator like
- Host:
- Rabbit
- Immunogen:
- The antiserum was produced against synthesized Acetyl-peptide derived from human BMAL1 around the Acetylation site of Lys538.
- Molecular Weight:
- 68 kDa
- Protein Details:
- Aryl hydrocarbon receptor nuclear translocator-like protein 1
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using correspond
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- -20Â[o]C
- Supplier:
- Aviva Systems Biology
- Type:
- Antibodies: Polyclonal Antibody
- Manufacturer's Data Sheet:html_datasheet.php
