POLD3 Antibody
Product Sizes
100 ug
£496.00
OAAF03578-100UG
About this Product
- SKU:
- OAAF03578
- Additional Names:
- DNA polymerase delta subunit 3;DNA polymerase delta subunit C;DNA polymerase delta subunit p66;DNA polymerase delta subunit p68;P66;P68;Pol delta C subunit (p66);polymerase (DNA) delta 3; accessory subunit;polymerase (DNA-directed); delta 3; accessory subunit;PPP1R128;protein phosphatase 1; regulatory subunit 128.
- Clonality:
- Polyclonal
- Extra Details:
- As a component of the trimeric and tetrameric DNA polymerase delta complexes (Pol-delta3 and Pol-delta4; respectively); plays a role in high fidelity genome replication; including in lagging strand synthesis; and repair. Required for optimal Pol-delta activity. Stabilizes the Pol-delta complex and plays a major role in Pol-delta stimulation by PCNA (PubMed:10219083; PubMed:10852724; PubMed:11595739; PubMed:16510448; PubMed:24035200). Pol-delta3 and Pol-delta4 are characterized by the absence or the presence of POLD4. They exhibit differences in catalytic activity. Most notably; Pol-delta3 shows higher proofreading activity than Pol-delta4 (PubMed:19074196; PubMed:20334433). Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro; Pol-delta3 may also be better suited to fulfill this task; exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap; while maintaining a nick that can be readily ligated (PubMed:24035200). Along with DNA polymerase kappa; DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation. In this context; POLD3; along with PCNA and RFC1-replication factor C complex; is required to recruit POLD1; the catalytic subunit of the polymerase delta complex; to DNA damage sites (PubMed:20227374). Under conditions of DNA replication stress; required for the repair of broken replication forks through break-induced replication (BIR) (PubMed:24310611). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites performed by Pol-delta4; independently of DNA polymerase zeta (REV3L) or eta (POLH). Facilitates abasic site bypass by DNA polymerase delta by promoting extension from the nucleotide inserted opposite the lesion (PubMed:19074196; PubMed:25628356; PubMed:27185888). Also involved in TLS; as a component of the POLZ complex. Along with POLD2; dramatically increases the efficiency and processivity of DNA synthesis of the minimal DNA polymerase zeta complex; consisting of only REV3L and REV7 (PubMed:24449906).
- Gene Details:
- DNA polymerase delta 3; accessory subunit
- Host:
- Rabbit
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human POLD3.
- Molecular Weight:
- 51 kDa
- Protein Details:
- DNA polymerase delta subunit 3
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- -20Â[o]C
- Supplier:
- Aviva Systems Biology
- Type:
- Antibodies: Polyclonal Antibody
- Manufacturer's Data Sheet:html_datasheet.php
