POLQ Antibody
Product Sizes
100 ug
£496.00
OAAF02053-100UG
About this Product
- SKU:
- OAAF02053
- Additional Names:
- DNA polymerase eta;DNA polymerase theta;epididymis secretory sperm binding protein;polymerase (DNA directed); theta;polymerase (DNA) theta;PRO0327.
- Clonality:
- Polyclonal
- Extra Details:
- DNA polymerase that promotes microhomology-mediated end-joining (MMEJ); an alternative non-homologous end-joining (NHEJ) machinery triggered in response to double-strand breaks in DNA (PubMed:25642963; PubMed:25643323). MMEJ is an error-prone repair pathway that produces deletions of sequences from the strand being repaired and promotes genomic rearrangements; such as telomere fusions; some of them leading to cellular transformation (PubMed:25642963; PubMed:25643323). POLQ acts as an inhibitor of homology-recombination repair (HR) pathway by limiting RAD51 accumulation at resected ends (PubMed:25642963). POLQ-mediated MMEJ may be required to promote the survival of cells with a compromised HR repair pathway; thereby preventing genomic havoc by resolving unrepaired lesions (By similarity). The polymerase acts by binding directly the 2 ends of resected double-strand breaks; allowing microhomologous sequences in the overhangs to form base pairs. It then extends each strand from the base-paired region using the opposing overhang as a template. Requires partially resected DNA containing 2 to 6 base pairs of microhomology to perform MMEJ (PubMed:25643323). The polymerase activity is highly promiscuous: unlike most polymerases; promotes extension of ssDNA and partial ssDNA (pssDNA) substrates (PubMed:18503084; PubMed:21050863; PubMed:22135286). Also exhibits low-fidelity DNA synthesis; translesion synthesis and lyase activity; and it is implicated in interstrand-cross-link repair; base excision repair and DNA end-joining (PubMed:14576298; PubMed:18503084; PubMed:19188258; PubMed:24648516). Involved in somatic hypermutation of immunoglobulin genes; a process that requires the activity of DNA polymerases to ultimately introduce mutations at both A/T and C/G base pairs (By similarity).
- Gene Details:
- DNA polymerase theta
- Host:
- Rabbit
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human POLQ.
- Molecular Weight:
- 197 kDa
- Protein Details:
- DNA polymerase theta
- Purification:
- The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- -20Â[o]C
- Supplier:
- Aviva Systems Biology
- Type:
- Antibodies: Polyclonal Antibody
- Manufacturer's Data Sheet:html_datasheet.php
