DH10B Electrocompetent E. coli Cells
Product Sizes
10 x 50 ul
EDH10B-201-10X50UL
About this Product
- SKU:
- EDH10B-201
- Application:
- Protein Engineering
- Extra Details:
- The DH10B electrocompetent E. coli cells are suitable for cloning and propagation of large size plasmids (10-150 kb in size). DH10B strain is known for stable replication of high-copy number plasmids and demonstrates improved cloning efficiency of mammalian DNA containing methylated cytosine or adenine. The high transformation efficiency makes them ideal for generating cDNA or genomic libraries. Electrocompetent DH10B E. coli cells are supplied as a pack of 10 convenient single use aliquots (50 ul/tube). Chemically Competent DH10B E. coli cells are also available. Bulk quantities and custom packaging are available at very competitive prices for all Competent Cells. Catalog # EDH10B-201 Genotype: F- mcrA D Delta(mrr-hsdRMS-mcrBC) F Phi80lacZD DeltaM15 D DeltalacX74 recA1 endA1 rpsL150(StrR) araD139 D Delta (ara, leu)7697 galU galK L Lambda- nupG Quality Control Testing: Transformations are performed using 50 ul aliquots of cells and 50 pg of pBR322 control plasmid. Features and Benefits: Transformation efficiency: ≥1x 1010cfu/ug pBR322 DNA Improved cloning of large plasmids and BACs due to constitutive deoxyribose synthesis Efficient transformation of both methylated and unmethylated DNA from genomic DNA, PCR, cDNA and many other sources due to the lack of methylation-dependent restriction systems Increased yield and quality of plasmid preparations due to mutation of nonspecific endonuclease I (endA1) Suitable for blue/white screening via lacZ-based A Alpha-complementation Recombination-deficient (recA1) Streptomycin resistant K12 Strain Economical price Storage: at -80°C.
- Shipping Conditions:
- Dry Ice
- Storage Conditions:
- -70[o]C
- Supplier:
- Amid Biosciences
- Type:
- Cells: Non-Human Cells
