BL21 (DE3) Electrocompetent E. coli Cells | High Level Recombinant Protein
Product Sizes
10 X 0.05 ml/tube
EBL21-201-10X0.05ML/TUBE
10 x 0.05 ml
EBL21-201-10X0.05ML
About this Product
- SKU:
- EBL21-201
- Application:
- Protein Engineering
- Extra Details:
- BL21(DE3) electrocompetent E. coli cells are suitable for transformation, high-level protein expression and production of recombinant proteins in bacterial systems. The strain lacks the lon and ompT proteases thus promoting stability of recombinant proteins. BL21(DE3) strain carries a copy of the T7 RNA polymerase under control of the IPTG inducible lacUV5 promoter, and as a result, is ideal for controlled expression of both E. coli and T7 promoter driven constructs. BL21 (DE3) electrocompetent cells are supplied as a pack of 10 single-use 50 ul/tube aliquots.The transformation efficiency is ≥ 1x108 cfu/ug pBR322 plasmid DNA. Catalog # EBL21-201 E. coli genotype: fhuA2 [lon] ompT gal (L Lambda DE3) [dcm] D DeltahsdS. [L Lambda DE3 = L Lambda sBamHIo D DeltaEcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 D Deltanin5] Quality Control Testing: Transformations are performed using 50 ul aliquots of cells and 50 pg of pUC19 control plasmid. Storage: Transfer the cells directly from the dry ice shipping container to the -80°C freezer. BL21 (DE3) Chemically Competent E.coli cells are also available.
- Shipping Conditions:
- Dry Ice
- Storage Conditions:
- -70[o]C
- Supplier:
- Amid Biosciences
- Type:
- Cells: Non-Human Cells
