oYo-Link[R] DBCO Antibody Labeling Reagent
Product Sizes
100 ug
£439.00
AT3003-100UG
5 x 100 ug
£1,241.00
AT3003-5X100UG
10 x 100 ug
£1,722.00
AT3003-10X100UG
About this Product
- SKU:
- AT3003
- Application:
- Conjugation/Labeling
- Buffer:
- PBS
- Conjugate:
- DBCO
- Concentration:
- 270 ug/ml
- Extra Details:
- NOTE: It is important to perform the click-reaction with Azide-x first, prior to antibody conjugation. UV illumination will damage the DBCO activity and thus antibody conjugation should only be performed after the click-reaction. Research Use Only Applications: Copper-free Click Chemistry oYo-Link[R] DBCO allows for the site-specific labeling of oYo-Link[R]-compatible antibodies with up to two dibenzocyclooctyne (DBCO) labels per antibody for subsequent copper-free click chemistry reactions. oYo-Link[R]-DBCO limits the attachment of the DBCO labels to the heavy chains of the antibody and therefore ensures that the DBCO label does not interfere with antibody binding. Furthermore, site-specific labeling limits 1-2 DBCO labels to be attached per antibody, providing precise antibody-conjugate ratios. The antibody labeling procedure simply requires mixing oYo-Link[R] -DBCO with the desired antibody followed by photocrosslinking with non-damaging black light (365 nm). Photocrosslinking can be carried in most buffers, including those containing amine-containing molecules (e.g. TRIS, glycine) or storage proteins (see Buffer Compatibility Table) . Requirements: To label your compatible antibodies with oYo-Link[R], you will need a Light Source emitting at 365nm (6-10W). Use your own Compatible Device from the list here or purchase AlphaThera's LED Photo-Crosslinking Device Here. Purchasing Information: For Credit Card purchases, proceed with checkout on website. To Request a Quote email sales@alphathera.com Technical Support: For questions concerning compatibility or click-chemistry procedure email support@alphathera.com
- Molecular Weight:
- 8 kDa
- Physical State:
- Dried pellet
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- -20[o]C Avoid freeze/thaw cycles.
- Supplier:
- AlphaThera
- Type:
- Buffers & General Consumables: Others