Study the Proteosome with Lifesensors
The Ubiquitin proteasome system (UPS) plays key roles in many aspects of cell physiology. Lysine 48 linked poly-ubiquitin chains dictate proteasomal degradation. Lysine 63 linked chains direct protein trafficking, autophagy and mitophagy. Many future drugs will come from Targeted Protein Degradation (TPD), such as molecular glues and PROTACs and modulators of E3 ubiquitin ligases. LifeSensors has the largest biologically active E3 ligase library.
Much of the scientific community use Luciferase reporter assays to discover TPD drugs. Reporter assays lead to artifacts that waste time and money. Monitoring direct ubiquitination of target protein by an E3 ligase is the simplest way to discover small molecules that ubiquitinate target proteins. LifeSensors has established a suite of assays and case reports for companies that prove successful discovery of molecular glues and PROTACs.
Proven Applications of TUBE embedded microtiter plates:
- Pan Poly-Ubiquitin TUBEs, captures all types of poly-ubiquitin: Discover MGs and PROTACs
- K48 Poly-Ubiquitin Selective TUBEs: Evaluation of proteasomal TPD via microtiter plate
- K63 Poly-Ubiquitin Selective TUBEs: Discover MGs that promote autophagy and mitophagy
- M1 Linear Poly-Ubiquitin Selective TUBE: Identify Inflammatory Target Degraders
- Ubiquitin Mass Spec Proteomics a detailed understanding of the MOA of a MG or PROTAC
The road to FDA approval is long, and it is essential to have accurate data to move to the clinic with confidence. Catch your hits at the source with natural ubiqutinomics.
