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10X TE pH 7.5

Product Sizes
1 l
MB-006-1L
About this Product
SKU:
MB-006
Additional Names:
10X TE pH 7.5 (100 mM Tris HCl 10 mM EDTA) Solution, 10X TE pH 7.5 DEPC TREATED Solution
Application:
Immunohistochemistry
Buffer:
See application note.
CE/IVD:
RUO
Concentration:
10X
Extra Details:
TE buffer is a commonly used buffer solution in molecular biology, especially in procedures involving DNA, cDNA or RNA. "TE" is derived from its components: Tris, a common pH buffer, and EDTA, a molecule that chelates cations like Mg2+. The purpose of TE buffer is to solubilize DNA or RNA, while protecting it from degradation.
Physical State:
Liquid
Shipping Conditions:
Ambient
Specificity:
This product was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Buffers & General Consumables: Others
10X TE pH 7.5 (100 mM Tris HCl 10 mM EDTA) DEPC Treated
Immunohistochemistry for MLH1 and MSH2 protein expression (hematoxylin, 400x). (a) Normal colorectal tissue with no antibody staining for MLH1 (negative control). (b) Normal colorectal tissue with MLH1 antibody staining (positive control). (c) Tumor with absence of MLH1 protein expression. (d) Tumor with MLH1 protein expression. (e) Normal colorectal tissue with no antibody staining for MSH2 (negative control). (f) Normal colorectal tissue with MSH2 antibody staining (positive control). (g) Tumor with absence of MSH2 protein expression. (h) Tumor with MSH2 protein expression. The slides underwent antigen retrieval with 10X Tris-ethylenediamine tetraacetic acid buffer, pH 7.5 (p/n MB-006) for 45 minutes on boiling water. Fig 2. PMID: 20012372.