Immunohistochemistry Workflow
Selecting the appropriate reagents for your IHC assay is an important part of optimizing your protocol. Vector Laboratories is your resource for premium labeling and detection products at each step of your IHC workflow.
Tissue Preparation | |
Antigen Retrieval | |
Quench / Block | BLOXALL™ Endogenous HRP and AP Blocking Solution Streptavidin / Biotin Blocking Kit |
Primary Antibody / Lectins | |
Secondary Antibody | ImmPRESS™ Polymer Reagents (HRP or AP) Mouse on Mouse (M.O.M.™) ImmPRESS™ Polymer Kit Mouse on Mouse (M.O.M.™) Basic Kit Biotinylated secondary antibodies Unconjugated secondary antibodies |
Tertiary Reagent | VECTASTAIN® ABC complex reagents ImmPRESS™ Excel Amplified Staining Kits |
Substrate / Chromogen | |
Counterstain | |
Coverslip / Mount | |
Visualise |