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PROGEN Hyperphage Portfolio

2BScientific Limited are proud to demonstrate our continued partnership with PROGEN, a trusted name in the field of antibodies and molecular biology. Based in Heidelberg, Germany, and ISO13485 certified, PROGEN is widely recognised for its reliable, high-quality tools designed to support cutting-edge research.

PROGEN’s Comprehensive Workflow 

The hyperphage production stage involves engineering E. coli to produce hyperphages lacking the pIII protein, using a helper phage vector (M13KO7ΔpIII). This system allows precise control over which proteins are displayed on the phage surface. 

In the hyperphage display stage, antibody fragments (scFv) are cloned into a phagemid vector containing a pIII gene. This construct is introduced into E. coli, which, when infected by the hyperphage, produces phages displaying the scFv–pIII fusion protein on their surface. 

During panning and elution, these phages are exposed to an antigen to identify strong binders. After washing, the bound phages are eluted using trypsin and re-amplified in E. coli. After 2–3 rounds, high-affinity binders are isolated and can be cloned into various formats for production of recombinant antibodies.

Hyperphage

Hyperphage

Elution


Why Choose PROGEN?

High quality – Thoroughly validated and trusted in laboratories worldwide. 

Scientifically credible – Widely cited and well established within the academic community. 

Expert support – Developed and supported by PhD-level scientists. 

Made in Germany – Precision-engineered for reliability and performance for Antibody Engineering. 

For more information on PROGEN's phage display portfolio, get in touch with us at sales@2BScientific — we’re here to help you find the right tools for your research. 

 

PROGEN Phage Display Product Portfolio 

Hyperphage M13 KO7ΔpIII

Vectors 

pOPE101

pSEX81

pAc-lambda-CH3

pAc-lambda-Fc

pAc-kappa-CH3

pAc-kappa-Fc

Library Primers 

Human IgG/IgM Primer Set

Mouse IgG Library Primer Set

ELISA 

Phage Titration ELISA