ImmunoChemistry Technologies / Apoptosis

Apoptosis is a form of programmed cell death that occurs in multicellular organisms. Biochemical events lead to characteristic cell changes (morphology) and death. These changes include blebbing, cell shrinkage, nuclear fragmentation chromatin condensation, DNA fragmentation, and mRNA decay. If you would like to learn more about it, please check the video below.

We now offer a range of apoptosis products for your research such as cell growth, immune surveillance and neoplastic development.

SKU	Product Name	Description
9124	Annexin V-FITC Apoptosis Assay	Detect apoptosis and membrane permeability with the Annexin V-FITC Apoptosis Assay Kit. This in vitro apoptosis assay employs the green fluorescent Annexin V-FITC reagent to label exposed, cell membrane-associated phosphatidylserine (PS) in cultured cell samples. Translocation of PS from the inner leaflet to the outer leaflet of the cell membrane is an early indicator of apoptosis induction. Detect membrane compromised cells, a trademark of late apoptosis or cell necrosis, with the live/dead stain, propidium iodide (PI). Analyze the fluorescent signals by flow cytometry.
9138	Bovine Annexin V-Fluorescein Apoptosis Assay	Detect apoptosis and membrane permeability with the Bovine Annexin V-Fluorescein Apoptosis Assay Kit. This in vitro apoptosis detection assay employs the green fluorescent Bovine Annexin V-Fluorescein reagent to label exposed, cell membrane-associated phosphatidylserine (PS) in cultured cell samples. Translocation of PS from the inner leaflet to the outer leaflet of the cell membrane is an early indicator of apoptosis Detect membrane compromised cells, a trademark of late apoptosis or cell necrosis, with the live/ dead stain, propidium iodide (PI). Analyze the fluorescent signals by flow cytometry induction.
9139	Canine Annexin V-Fluorescein Apoptosis Assay	Detect apoptosis and membrane permeability with the Canine Annexin V-Fluorescein Apoptosis Assay Kit. Translocation of PS from the inner leaflet to the outer leaflet of the cell membrane is an early indicator of apoptosis. This in vitro apoptosis assay employs the green fluorescent Canine Annexin V-Fluorescein reagent to label exposed, cell membrane-associated phosphatidylserine (PS) in cultured cell samples. Detect membrane compromised cells, a trademark of late apoptosis or cell necrosis, with the live/dead stain, propidium iodide (PI). Analyze the fluorescent signals by flow cytometry.
9140	Chicken Annex- in V-Fluores- cein Apoptosis Assay	Detect apoptosis and membrane permeability with the Chicken Annexin V-Fluorescein Apoptosis Assay Kit. Translocation of PS from the inner leaflet to the outer leaflet of the cell membrane is an early indicator of apoptosis. This in vitro apoptosis assay employs the green fluorescent Chicken Annexin V-Fluorescein reagent to label exposed, cell membrane associated phosphatidylserine (PS) in cultured cell samples. Detect membrane compromised cells, a trademark of late apoptosis or cell necrosis, with the live/dead stain, propidium iodide (PI). Analyze the fluorescent signals by flow cytometry.
9141	Equine Annexin V-Fluorescein Apoptosis Assay	Detect apoptosis and membrane permeability with the Equine Annexin V-Fluorescein Apoptosis Assay Kit. Translocation of PS from the inner leaflet to the outer leaflet of the cell membrane is an early indicator of apoptosis. This in vitro apoptosis assay employs the green fluorescent Equine Annexin V-Fluorescein reagent to label exposed, cell membrane associated phosphatidylserine (PS) in cultured cell samples. Detect membrane compromised cells, a trademark of late apoptosis or cell necrosis, with the live/dead stain, propidium iodide (PI). Analyze the fluorescent signals by flow cytometry.
9142	Swine Annexin V-Fluorescein Apoptosis Assay	Detect apoptosis and membrane permeability with the Swine Annexin V-Fluorescein Apoptosis Assay Kit. Translocation of PS from the inner leaflet to the outer leaflet of the cell membrane is an early indicator of apoptosis. This in vitro apoptosis assay employs the green fluorescent Swine Annexin V-Fluorescein reagent to label exposed, cell membrane-associated phosphatidylserine (PS) in cultured cell samples. Detect membrane compromised cells, a trademark of late apoptosis or cell necrosis, with the live/dead stain, propidium iodide (PI). Analyze the fluorescent signals by flow cytometry.
6210	Camptothecin	Camptothecin, a cytotoxic plant alkaloid with antitumor properties, is a prototypic DNA topoisomerase I inhibitor. Use camptothecin to create positive controls in apoptosis detection experiments.
6212	Staurosporine	Staurosporine, isolated from Streptomyces staurospores, is a protein kinase C inhibitor. Staurosporine induces DNA fragmen- tation and apoptosis at 1 µM in >4 hours.
50	FAM-FLICA® Poly Caspase Assay	This in vitro assay employs the fluorescent, cell-membrane per- meant, poly caspase-inhibitor probe, FAM-VAD-FMK. This probe contains the generic tripeptide caspase targeting sequence (VAD) that enables the detection of most intracellular caspases in living cells. Analyze samples using fluorescence microscopy, a fluores- cence plate reader, or flow cytometry.
6354	FAM-VAD-OPH I in vitro Apop- tosis Detection Reagent	A novel set of inhibitor reagents that employ an O-phenoxy (OPH) reactive group instead of an FMK group. In a manner analogous to the FMK class of cysteine reactive compounds, the OPH inhibitors form a stable covalent thioether adduct with the reactive SH-site of caspase enzymes present in apoptotic cells. These reagents are used as simple and reliable methods for screening apoptosis in live cells. FAM-VAD-OPH I [5-FAM-Val-Ala-Asp(OMe)-2,6-diflu- orophenoxy-methylketone poly caspase inhibitor] can be used with a fluorescence microscope, fluorescence plate reader, or flow cytometry.
6355	FAM-VAD-OPH II in vitro Apop- tosis Detection Reagent	A novel set of inhibitor reagents that employ an O-phenoxy (OPH) reactive group instead of an FMK group. In a manner analogous to the FMK class of cysteine reactive compounds, the OPH inhibitors form a stable covalent thioether adduct with the reactive SH-site of caspase enzymes present in apoptotic cells. These reagents are used as simple and reliable methods for screening apopto- sis in live cells. FAM-VAD-OPH II [5-FAM-Val-Ala-Asp(OMe)-Oph (2,6-diF) poly caspase inhibitor] can be used with a fluorescence microscope, fluorescence plate reader, or flow cytometry.
9120	FLICA® 660 Poly Caspase Assay	This in vitro assay employs the fluorescent cell-membrane per- meant, poly caspase-inhibitor probe, 660-VAD-FMK. This probe contains the generic tripeptide caspase targeting sequence (VAD) that enables the detection of most intracellular caspases in living cells. Analyze the fluorescent signal using fluorescence micros- copy or flow cytometry.
916	SR-FLICA® Poly Caspase Assay	This in vitro assay employs the fluorescent cell-membrane permeant, poly caspase-inhibitor probe, SR-VAD-FMK. This probe contains the generic tripeptide caspase targeting sequence (VAD) that enables the detection of most intracellular caspases in living cells. Analyze samples using fluorescence microscopy, a fluores- cence plate reader, or flow cytometry.
6357	SR-VAD-OPH in vitro Apopto- sis Detection Reagent	A novel set of inhibitor reagents that employ an O-phenoxy (OPH) reactive group instead of an FMK group. In a manner analogous to the FMK class of cysteine reactive compounds, the OPH inhibi- tors form a stable covalent thioether adduct with the reactive SH-site of caspase enzymes present in apoptotic cells. These reagents are used as simple and reliable methods for screening apoptosis in live cells. SR-VAD-OPH [SR-Val-Ala-Asp(OMe)-Oph (2,6-diF) poly caspase inhibitor] can be used with a fluorescence microscope, fluorescence plate reader, or flow cytometry.
980	FAM-FLIVO® In vivo Poly Cas- pase Assay	FLIVO (FLuorescence in vIVO) is a powerful method for assess- ing caspase activity in vivo. FAM-FLIVO poly caspase probes are non-cytotoxic, cell-permeant fluorescent inhibitors of caspases optimized for use in whole live animals. ICT’s FAM-FLIVO poly cas- pase inhibitor probe contains the preferred binding sequence for most caspases (Val-Ala-Asp or VAD). This preferred poly caspase tripeptide binding sequence is labeled with a carboxyfluorescein (FAM) dye and a fluoromethyl ketone (FMK) reactive entity.
982	SR-FLIVO® In vivo Poly Cas- pase Assay	FLIVO (FLuorescence in vIVO) is a powerful method for assessing caspase activity in vivo. SR-FLIVO poly caspase probes are non-cy- totoxic, cell-permeant fluorescent inhibitors of caspases optimized for use in whole live animals. ICT’s SR-FLIVO poly caspase inhibitor probe contains the preferred binding sequence for most caspases (Val-Ala-Asp or VAD). This preferred poly caspase tripeptide bind- ing sequence is labeled with a sulforhodamine B (SR) dye and a fluoromethyl ketone (FMK) reactive entity.
9112	NIR-FLIVO® 690 Tracer In vivo Assay	FLIVO (FLuorescence in vIVO) is a powerful method for non-inva- sive detection of caspase activity in vivo. NIR-FLIVO 690 Tracers are non-cytotoxic, cell-permeant fluorescent inhibitors of caspases optimized for use in whole live animals. ICT’s NIR-FLIVO 690 Tracer probe contains the preferred binding sequence for most caspases (Val-Ala-Asp or VAD). This preferred poly caspase tripeptide bind- ing sequence is labeled with Dylight® 690 dye and a fluoromethyl ketone (FMK) reactive entity. FLIVO® is a registered trademark of ICT. Dylight® is a registered trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
9113	NIR-FLIVO® 690 Free Dye Control Assay	ICT’s near-infrared (NIR)-FLIVO Tracers are used in conjunction with NIR-FLIVO Free Dye Control Assays. The NIR-FLIVO Free Dye Control Assay uses the NIR-FLIVO Free Dye Control reagent (Dylight® 690 Free Dye). When injected, both the Free Dye Control and the Tracer reagents will generate a fluorescent signal, but only the Tracer should bind to active caspases and remain inside an apoptotic cell, while signal associated with the Free Dye Control represents background noise. FLIVO® is a registered trademark of ICT. Dylight® is a registered trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
9114	NIR-FLIVO® 747 Tracer In vivo Assay	FLIVO (FLuorescence in vIVO) is a powerful method for non-inva- sive detection of caspase activity in vivo. NIR-FLIVO 747 Tracers are non-cytotoxic, cell-permeant fluorescent inhibitors of caspases optimized for use in whole live animals. ICT’s NIR-FLIVO 747 Tracer probe contains the preferred binding sequence for most caspases (Val-Ala-Asp or VAD). This preferred poly caspase tripeptide bind- ing sequence is labeled with Dylight® 747 dye and a fluoromethyl ketone (FMK) reactive entity. FLIVO® is a registered trademark of ICT. Dylight® is a registered trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
9115	NIR-FLIVO® 747 Free Dye Control Assay	ICT’s near-infrared (NIR)-FLIVO® Tracers are used in conjunc- tion with NIR-FLIVO Free Dye Control Assays. The NIR-FLIVO Free Dye Control Assay uses the NIR-FLIVO Free Dye Control reagent (Dylight® 747 Free Dye). When injected, both the Free Dye Control and the Tracer reagents will generate a fluorescent signal, but only the Tracer should bind to active caspases and remain inside an apoptotic cell, while signal associated with the Free Dye Control represents background noise. FLIVO® is a registered trademark of ICT. Dylight® is a registered trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
918	FAM-FLICA® Caspase-2 Assay	This in vitro assay employs the fluorescent cell-membrane permeant, caspase-2 inhibitor probe, FAM-VDVAD-FMK. This probe contains the preferred pentapeptide caspase 2 targeting sequence (VDVAD) that enables the detection of active caspase-2 enzyme in living cells. Analyze samples using fluorescence mi- croscopy, a fluorescence plate reader, or flow cytometry.
93	FAM-FLICA® Caspase-3/7 Assay	This in vitro assay employs the fluorescent cell-membrane permeant, caspase-3/7 inhibitor probe, FAM-DEVD-FMK. This probe contains the preferred tetrapeptide caspase-3/7 targeting sequence (DEVD) that enables the detection of active caspase 3 and 7 enzymes in living cells. Analyze samples using fluorescence microscopy, a fluorescence plate reader, or flow cytometry.
6356	FAM-DEVD- OPH in vitro Apoptosis Detection Reagent	A novel set of inhibitor reagents that employ an O-phenoxy (OPH) reactive group instead of an FMK group. In a manner analogous to the FMK class of cysteine reactive compounds, the OPH inhibitors form a stable covalent thioether adduct with the reactive SH-site of caspase enzymes present in apoptotic cells. These reagents are used as simple and reliable methods for screening apoptosis in live cells. FAM-DEVD-OPH [5-FAM-Asp-Glu-Val-Asp(OMe)-Oph (2,6-diF) caspase 3/7 inhibitor] can be used with a fluorescence microscope, fluorescence plate reader, or flow cytometry.
9125	FLICA 660® Caspase-3/7 Assay	This in vitro assay employs the fluorescent cell-membrane permeant, caspase-3/7 inhibitor probe, 660-DEVD-FMK. This probe contains the preferred tetrapeptide caspase-3/7 targeting sequence (DEVD) that enables the detection of active caspase 3 and 7 enzyme in living cells. Analyze the fluorescent signal using fluorescence microscopy or flow cytometry.
936	Magic Red® Caspase-3/7 Assay	Detect caspase 3 and 7 activity in real time with the Magic Red Caspase-3/7 Assay. The Magic Red fluorogenic substrate contains the preferred caspase-3/7 targeting sequences (DEVD). It fluoresces upon cleavage by active caspase 3 and 7 enzymes. Analyze the fluorescent signal using fluorescence microscopy, a fluorescence plate reader, or flow cytometry.
931	SR-FLICA® Caspase-3/7 Assay	This in vitro assay employs the fluorescent cell-membrane permeant, caspase-3/7 inhibitor probe, SR-DEVD-FMK. This probe contains the preferred tetrapeptide caspase-3/7 targeting sequence (DEVD) that enables the detection of active caspase 3 and 7 enzymes in living cells. Analyze the fluorescent signal using fluorescence microscopy, a fluorescence plate reader, or by flow cytometry.
95	FAM-FLICA® Caspase-6 Assay	This in vitro assay employs the fluorescent cell-membrane permeant, caspase-6 inhibitor probe, FAM-VEID-FMK. This probe contains the preferred tetrapeptide caspase-6 targeting sequence (VEID) that enables the detection of active caspase-6 enzyme in living cells. Analyze samples using fluorescence mi- croscopy, a fluorescence plate reader, or flow cytometry.
99,910	FAM-FLICA® Caspase-8 Assay	This in vitro assay employs the fluorescent cell-membrane permeant, caspase-8 inhibitor probe, FAM-LETD-FMK. This probe contains the preferred tetrapeptide caspase-8 targeting sequence (LETD) that enables the detection of active caspase-8 enzyme in living cells. Analyze samples using fluorescence mi- croscopy, a fluorescence plate reader, or flow cytometry.
9149,9150	SR-FLICA® Caspase-8 Assay	This in vitro assay employs the fluorescent, cell-membrane per- meant, caspase-8 inhibitor probe, SR-LETD-FMK. This probe con- tains the preferred tetrapeptide caspase-8 targeting sequence (LETD) that enables the detection of active caspase-8 enzyme in living cells. Analyze samples using fluorescence microscopy, a fluorescence plate reader, or flow cytometry.
912,913	FAM-FLICA® Caspase-9 Assay	This in vitro assay employs the fluorescent cell-membrane permeant, caspase-9 inhibitor probe, FAM-LEHD-FMK. This probe contains the preferred tetrapeptide caspase-9 targeting sequence (LEHD) that enables the detection of active caspase-9 enzyme in living cells. Analyze samples using fluorescence microscopy, a fluorescence plate reader, or flow cytometry.
960,961	SR-FLICA® Caspase-9 Assay	This in vitro assay employs the fluorescent cell-membrane perme- ant, caspase-9 inhibitor probe, SR-LEHD-FMK. This probe contains the preferred tetrapeptide caspase-9 targeting sequence (LEHD) that enables the detection of active caspase-9 enzyme in living cells. Analyze samples using fluorescence microscopy, a fluores- cence plate reader, or flow cytometry.
502,503	FAM-FLICA® Caspase-10 Assay	This in vitro assay employs the fluorescent cell-membrane per- meant, caspase-10 inhibitor probe, FAM-AEVD-FMK. This probe contains the preferred tetrapeptide caspase-10 targeting sequence (AEVD) that enables the detection of active caspase-10 enzyme in living cells. Analyze samples using fluorescence microscopy, a fluorescence plate reader, or flow cytometry.
9147,9148	Necrosis vs Apoptosis Assay	Simultaneously detects both apoptosis associated cytotoxicity events and cell death due to necrosis. This simple and straightfor- ward tool allows researchers to understand the overall health- status of their cell populations. In addition, this kit is useful to re- searchers investigating the effect of their novel drug or therapeutic as it allows them to assess their experimental outcome and evalu- ate the overall treatment of the cells. Analyze using fluorescence microscopy or flow cytometry.
949,950	FLISP FAM-Leu-CMK Serine Protease Assay	FLISP probes interact with active catalytic sites of chymotrypsin- like proteases, labeling cells with greater quantities of active chymotrypsin-like enzyme activity with a greater fluorescence po- tential than those that are not upregulated. Analyze your samples using a flow cytometer, fluorescence microscope, or fluorescence plate reader.
967,968	FLISP FAM-Leu-DAP Serine Protease Assay	FLISP probes interact with active catalytic sites of chymotrypsin- like proteases, labeling cells with greater quantities of active chymotrypsin-like enzyme activity with a greater fluorescence po- tential than those that are not upregulated. Analyze your samples using a flow cytometer, fluorescence microscope, or fluorescence plate reader.
945,946	FLISP FAM-Phe-CMK Serine Protease Assay	FLISP probes interact with active catalytic sites of chymotrypsin- like proteases, labeling cells with greater quantities of active chymotrypsin-like enzyme activity with a greater fluorescence po- tential than those that are not upregulated. Analyze your samples using a flow cytometer, fluorescence microscope, or fluorescence plate reader.
984,985	FLISP FAM-Phe-DAP Serine Protease Assay	FLISP probes interact with active catalytic sites of chymotrypsin- like proteases, labeling cells with greater quantities of active chymotrypsin-like enzyme activity with a greater fluorescence po- tential than those that are not upregulated. Analyze your samples using a flow cytometer, fluorescence microscope, or fluorescence plate reader.
965,966	FLISP FAM- Spacer-Leu- CMK Serine Protease Assay	FLISP probes interact with active catalytic sites of chymotrypsin- like proteases, labeling cells with greater quantities of active chymotrypsin-like enzyme activity with a greater fluorescence po- tential than those that are not upregulated. Analyze your samples using a flow cytometer, fluorescence microscope, or fluorescence plate reader.
963,964	FLISP FAM- Spacer-Phe- CMK Serine Protease Assay	FLISP probes interact with active catalytic sites of chymotrypsin- like proteases, labeling cells with greater quantities of active chymotrypsin-like enzyme activity with a greater fluorescence po- tential than those that are not upregulated. Analyze your samples using a flow cytometer, fluorescence microscope, or fluorescence plate reader.
955,956	FLISP SR101- Leu-CMK Serine Protease Assay	FLISP probes interact with active catalytic sites of chymotrypsin- like proteases, labeling cells with greater quantities of active chymotrypsin-like enzyme activity with a greater fluorescence po- tential than those that are not upregulated. Analyze your samples using a flow cytometer, fluorescence microscope, or fluorescence plate reader.
951, 952	FLISP SR101- Phe-CMK Serine Protease Assay	FLISP probes interact with active catalytic sites of chymotrypsin- like proteases, labeling cells with greater quantities of active chymotrypsin-like enzyme activity with a greater fluorescence po- tential than those that are not upregulated. Analyze your samples using a flow cytometer, fluorescence microscope, or fluorescence plate reader.
N/A	Granzyme B	Highly active Granzyme B expressed in yeast from a human cDNA. Study enzyme regulation and kinetics, cleave target substrates, and screen for inhibitors.
6213	Tumor Necrosis Factor alpha	Tumor Necrosis Factor- Alpha (TNF-α), also known as TNF ligand superfamily member 2 (TNFSF2) and cachectin, is a human recombinant protein that can be used in a variety of applications including cytotoxicity, cell proliferation assays, apoptosis, and viral protection. This product is best used for inducing apoptosis to create a positive control.
6214	Tumor Necrosis Factor beta	Tumor Necrosis Factor – Beta (TNF-β), also known as lympho- toxin-alpha is a human recombinant protein that can be used in a variety of applications including cytotoxicity, cell proliferation assays, and apoptosis. This product is best used for inducing apoptosis to create a positive control.

Basket

You currently have no items in your basket.

Apoptosis

We now offer a range of apoptosis products for your research such as cell growth, immune surveillance and neoplastic development.