ichorbio's Complete In Vivo Antibody Dosing Guide
Our colleagues at ichorbio have essential advice for end user on how to select the ideal antibody dose for mouse model studies.
Are you unsure of the perfect antibody dose that your research requires?
Check out the product data below or ichorbio's Antibody Dosing Guide!
Checkpoint Blockade Antibodies
Product: RMP-14 (Anti-PD-1)
Target: PD-1 (CD279)
Species: Mouse
Standard Dose Range: 200-500 μg per mouse Recommended Route: Intraperitoneal injection
Optimal Dosing Schedule: Every 3-4 days
Applications: Cancer immunotherapy studies (PD-1 checkpoint blockade); reinvigorating exhausted T cells in chronic infection models
Published Examples: Often used at 200 μg per dose in syngeneic tumour models including MC38 and B16 melanoma
Product: 10F.9G2 (Anti-PD-L1)
Target: PD-L1 (B7-H1, CD274)
Species: Mouse
Standard Dose Range: 100-250 μg per mouse
Recommended Route: Intraperitoneal injection
Optimal Dosing Schedule: 2-3 times per week
Applications: Cancer immunotherapy studies (PD-L1 blockade); also used in infection models to enhance T-cell responses by blocking PD-1/PD-L1 interaction
Product: 29F.1A12 (Anti PD-1)
Target: PD-1 (CD279)
Species: Mouse
Standard Dose Range: 100-200 μg per mouse (~5 mg/kg)
Recommended Route: Intraperitoneal injection
Optimal Dosing Schedule: Given 3 times at 3-day intervals
Applications: Cancer immunotherapy (PD-1 blockade); used in tumour models to stimulate anti-tumour T-cell activity, often in combination with other treatments
Product: 9H10 (Anti-CTLA-4)
Target: CTLA-4 (CD152)
Species: Mouse
Standard Dose Range: 100-200 μg per mouse
Recommended Route: Intraperitoneal injection
Optimal Dosing Schedule: Every ~3 days
Applications: Cancer immunotherapy (CTLA-4 blockade in tumor models to enhance T-cell activation; often combined with anti-PD-1/PD-L1 therapy; mimics ipilimumab's effect)
Product: 9D9 (Anti-CTLA-4)
Target: CTLA-4 (CD152)
Species: Mouse
Standard Dose Range: 100-250 μg per mouse
Recommended Route: Intraperitoneal injection (also tested intratumoral)
Optimal Dosing Schedule: Every 3 days
Applications: Cancer immunotherapy (CTLA-4 blockade); notably, clone 9D9 can deplete intertumoral Tregs due to its mouse IgG2b Fc, enhancing anti-tumour immunity
Immune Cell Depleting Antibodies
Product: GKL5 (Anti-CD4)
Target: CD4 (T helper cell marker)
Species: Mouse
Standard Dose Range: 200-250 μg per mouse
Recommended Route: Intraperitoneal injection
Optimal Dosing Schedule: 2-3 times per week
Applications: CD4⁺ T cell depletion studies - used to test CD4 T cell requirement in autoimmune disease models, infection immunity, tumor rejection, and transplant tolerance
Product: 2.43 (Anti-CD8)
Target: CD8α (Cytotoxic T cell marker)
Species: Mouse
Standard Dose Range: 250 μg per mouse
Recommended Route: Intraperitoneal injection
Optimal Dosing Schedule: 2-3 times per week
Applications: CD8⁺ T cell depletion - eliminates cytotoxic T lymphocytes to assess their role in tumour immunity, viral clearance, graft rejection, etc. Often used alongside GK1.5 to deplete all T cell subsets
Product: RB6-8C5 (Anti-Gr-1)
Target: Gr-1 (Ly6G/Ly6C on neutrophils)
Species: Mouse
Standard Dose Range: 200-250 μg per mouse
Recommended Route: Intraperitoneal injection
Optimal Dosing Schedule: Every 2-3 days for sustained neutrophil depletion
Applications: Neutrophil/granulocyte depletion - used to study neutrophils' role in inflammation, infection, and cancer models
Product: 1A8 (Anti-Ly6G)
Target: Ly6G (neutrophil-specific marker)
Species: Mouse
Standard Dose Range: 100-250 μg per mouse
Recommended Route: Intraperitoneal injection
Optimal Dosing Schedule: 3 times per week or every 3 days
Applications: Neutrophil-specific depletion - more selective than RB6-8C5; used in tumour immunotherapy studies, infection models, and inflammation assays
Product: PK136 (Anti-NK1.1)
Target: NK1.1 (NK cell marker, also known as NKR-P1C/CD161)
Species: Mouse (NK1.1 is present in C57BL/6 and related strains)
Standard Dose Range: 200-300 μg per mouse
Recommended Route: Intraperitoneal injection
Optimal Dosing Schedule: 1-3 times per week as needed
Applications: NK cell depletion - eliminates natural killer cells to assess their function in tumour metastasis control, viral infections, and immune regulation
Immune Activating Antibodies
Product: 145-2C11 (Anti-CD3c)
Target: CD3ε (T-cell receptor complex)
Species: Mouse
Standard Dose Range: 5-50 μg per mouse
Recommended Route: Intravenous injection (tail vein)
Optimal Dosing Schedule: 5 μg/day for 5 days or single 10 μg dose
Applications: Polyclonal T-cell activation in vivo, causing transient T-cell proliferation and cytokine release; used to induce T-cell depletion/activation-induced cell death and immune tolerance in autoimmunity
Product: FGK4.5/FGK45 (Anti-CD40)
Target: CD40 (costimulatory receptor on APCs)
Species: Mouse
Standard Dose Range: 50-300 μg per mouse
Recommended Route: Intraperitoneal or intravenous injection
Optimal Dosing Schedule: Single or consecutive daily doses
Applications: Agonistic CD40 activation to stimulate antigen-presenting cells; used as immune adjuvant in cancer immunotherapy, in vaccine models, and in autoimmune disease models
