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Assay Kits & ELISAs

ImmPRESS[R]-HRP Goat Anti-Rabbit IgG Polymer Detection Kit, Peroxidase

Product Sizes
15 ml
MP-7451-15ML
50 ml
MP-7451-50ML
About this Product
SKU:
MP-7451
Additional Names:
ImmPRESS Enzyme Polymer Reagents [Detection Systems ]
Application:
IHC-Frozen, IHC-Paraffin, Immunocytochemistry, Immunohistochemistry
Conjugate:
HRP
Extra Details:
Features: High sensitivity Low background One-step detection Micropolymer technology Enhanced accessibility to nuclear and membrane antigens Ready-to-use in a convenient dropper bottle Shorter assay time Simplified multiple labeling Kit Contents: ImmPRESS[TM] (Peroxidase) Polymer Anti-Rabbit IgG Reagent (made in goat, ready-to-use) 2.5% Normal Goat Serum for blocking (ready-to-use) The ImmPRESS[TM] (15 ml) Kit will stain approximately 75-150 sections and the ImmPRESS[TM] (50 ml) Kit will stain approximately 250-500 sections.The ImmPRESS[TM] polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. This unique micropolymer of highly active enzyme (peroxidase or alkaline phosphatase) is attached to our affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background. The peroxidase micropolymers of the ImmPRESS[TM] HRP polymer reagent limit steric interference and provide enhanced accessibility to the target, avoiding the disadvantages of other polymer systems that use large dextrans or other macromolecules as backbones. The result is crisp, strong staining of antibody targets, especially nuclear and membrane antigens (such as Ki67, estrogen receptor, bcl-2, CD3, CD8 and CD10) and greater sensitivity than other polymer systems. The staining procedure is simple as shown in the diagram below. Following a blocking step with the diluted normal horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS[TM] Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with the peroxidase substrate of choice.
Host:
Goat
Immunogen:
IgG
Reactivities:
Rabbit
Shipping Conditions:
Ambient
Storage Conditions:
2-8[o]C
Supplier:
Vector Laboratories
Type:
Assay Kits: IHC Kits
What are the advantages of using IMMPRESS Excel Kits compared with one-step peroxidase polymer detection systems:
The ImmPRESS Excel kits are a two-step peroxidase polymer based detection system that include an unconjugated amplifier antibody. This intermediate amplifier antibody increases sensitivity of the assay at least three- to four-fold over that of a one-step polymer based system, by facilitating the introduction of more peroxidase enzyme at the site of specific antigen localization. This increase in sensitivity would be advantageous in instances of weak antigen expression, and to further dilute out an expensive primary antibody. The ImmPRESS Excel Amplifier kits are presented as a complete kit format that include enzyme quench, blocking serum and ImmPACT® DAB EqV peroxidase substrate, in addition to the amplifier antibody and defined ImmPRESS polymer secondary antibody. However, as the two published references below indicate, this format is still modular and allows for the substitution of different detection reagents. These references describe using ImmPACT NovaRED™ peroxidase substrate in combination with the ImmPRESS Excel Amplifier kits. Oncogenesis (2017) 6, e293; doi:10.1038/oncsis.2016.82 Tan, E.M.S., et al (2017) Front. Med. 4:162 (doi: 10.3389/fmed.2017.00162)
Can ImmPRESS reagents be applied to an automated system, and do you have any guidelines or procedures?:
Yes, we conducted studies on the compatibility of the ImmPRESS HRP polymer reagents with three commercially available autostainers (Agilent/Dako Autostainer Plus, Leica Bond Rx, and Ventana Discovery Ultra). We showed that our reagents are suitable for IHC detection on each of these platforms. The ImmPRESS polymer reagents and enzyme substrates generated equivalent IHC staining results compared to reagents from the instrument manufacturer. Some modifications in the protocol were performed to optimize the signal-to-noise ratio, such as a shorter incubation time for the ImmPRESS polymer and increase in the number of buffer washes following polymer incubation. Please see the following application note for more details about each automated platform:https://vectorlabs.com/media/contentmanager/content/docs/brochures/VL_LIT3023_ImmPRESS_AppNo