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Antigen Unmasking Solution, Citric Acid Based

Product Sizes
250 ml
H-3300-250ML
Academic Pricing
Please contact us for academic pricing.
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About this Product
SKU:
H-3300
Additional Names:
Histology Reagents [Default] Epitope Retrieval, Antigen Unmasking
Application:
IHC-Frozen, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
Extra Details:
Vector Laboratories Antigen Unmasking Solutions are highly effective at revealing antigens in formalin-fixed, paraffin embedded tissue sections when used in combination with a high temperature treatment procedure. These solutions for antigen retrieval are available in two formulations. The citrate-based solution (H-3300) is pH 6.0; the Tris-based solution (H-3301) is pH 9.0. Both solutions are supplied as convenient 100x concentrated stocks, sufficient for preparation of 25 L of working solution.
Shipping Conditions:
Ambient
Storage Conditions:
2-8[o]C
Supplier:
Vector Laboratories
Type:
Buffers & General Consumables: Others
I have a bottle of the antigen unmasking solution H-3300. It has been stored in the fridge for a few weeks. When I opened it, it contained some precipitate. What is it and can it affect performance?:
Product H-3300 is supplied as a highly concentrated (100x) salt solution. It is recommended to be stored in the fridge. In some cases, over time with cold storage, some salts may come out of solution and appear as particulate or precipitated material. Our recommendation is to gently warm the bottle in a warm water bath to re-dissolve the precipitate. Usually 30 min at 35 °C would be sufficient. Once re-dissolved, an aliquot can be drawn from this solution and diluted according to the instructions. Re-dissolving the precipitate maintains the desired pH and salt concentration for optimal performance.
What are the components in the antigen unmasking solution, citrate buffer pH 6, H-3300?:
The stock solution of H-3300 is supplied as 1M citrate. Once diluted according to the instructions, the working concentration would be 0.01 M citrate. It is essentially a saturated concentrated salt solution.