AZDye 405 Picolyl Azide
Product Sizes
1 mg
CCT-1308-1MG
5 mg
CCT-1308-5MG
25 mg
CCT-1308-25MG
About this Product
- SKU:
- CCT-1308
- Application:
- Conjugation/Labeling
- Conjugate:
- AZDye™ 405
- Extra Details:
- AZDye™ 405 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported. In addition, the use picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling, significantly improving biocompatibility of CuAAC labeling protocol. In summary, the introduction of a copper-chelating motif into azide probe leads to a substantial increase in the sensitivity and reduced cell toxicity of CuAAC detection alkyne-tagged biomolecules. This will be of special value for the detection of low abundance targets or living system imaging. AZDye™ 405 is structurally similar and spectrally is almost identical to DyLight® 405, Alexa Fluor® 405, CF® 405S Dye, or any other sulfonated pyrene based fluorescent dyes.
- Molecular Weight:
- 718.68 (protonated) 1022.26 (triethylammonium salt)
- Shipping Conditions:
- Ambient
- Storage Conditions:
- -20[o]C desiccated. Avoid freeze/thaw cycles.
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Fluorophores
- Manufacturer's Data Sheet:AZDye-405-Picolyl-Azide
