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Wisteria Floribunda Lectin (WFA, WFL), Biotinylated

Product Sizes
2mg
£265.00
B-1355-2MG
About this Product
SKU:
B-1355
Additional Names:
Lectins & Glycobiology Reagents [Lectins ], Japanese Wisteria Lectin, N-acetylgalactosamine binding lectin
Application:
Immunohistochemistry, IHC-Frozen, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, ELISPOT
Conjugate:
Biotin
Extra Details:
The binding specificity of Wisteria floribunda lectin (WFL) is not completely clear but this lectin appears to preferentially bind carbohydrate structures terminating in N-acetylgalactosamine linked α or β to the 3 or 6 position of galactose. This lectin has been used to fractionate lymphocyte populations, and although not mitogenic, elicits the production of lymphokines from murine splenocytes.Biotinylated WFL has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium sodium azide.This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative. Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine
Shipping Conditions:
Ambient
Storage Conditions:
2-8 [o]C; Store frozen for long term storage
Supplier:
Vector Laboratories