AZDye 488 Biotin
Product Sizes
1 mg
£294.00
CCT-1395-1MG
5 mg
£815.00
CCT-1395-5MG
About this Product
- SKU:
- CCT-1395
- Application:
- Conjugation/Labeling
- Conjugate:
- AZDye™ 488, Biotin
- Extra Details:
- AZDye™ 488 Biotin can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. This reagent overcomes major shortcomings of commonly used Biotin-4-fluorescein – poor solubility in aqueous media and pH dependent absorbance and emission. AZDye™ 488 Biotin is a water soluble reagent and its green fluorescence is pH independent from pH 4 to pH 10. A flexible PEG3 spacer between biotin moiety and fluorescent tag minimize steric hindrance involved in binding to avidin, streptavidin or neutravidin. AZDye™ 488 is a bright, and highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of blue dyes are best suited to direct imaging of low-abundance targets. AZDye™ 488 is structurally identical to Alexa Fluor® 488. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, Alexa Fluor® 488, and CF® 488A. DyLight® and Alexa Fluor® are a registered trademark of Thermo Fisher Scientific.
- Molecular Weight:
- 935.00 (protonated)
- Shipping Conditions:
- Ambient
- Storage Conditions:
- -20[o]C desiccated. Avoid freeze/thaw cycles.
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Fluorophores
- Manufacturer's Data Sheet:AZDye-488-Biotin
