Cy5.5 Picolyl Azide
Product Sizes
1 mg
£283.00
CCT-1182-1MG
5 mg
£709.00
CCT-1182-5MG
25 mg
£1935.00
CCT-1182-25MG
About this Product
- SKU:
- CCT-1182
- Application:
- Conjugation/Labeling
- Conjugate:
- Cyanine 5
- Extra Details:
- Cy5.5 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported. In addition, the use picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling, significantly improving biocompatibility of CuAAC labeling protocol. In summary, the introduction of a copper-chelating motif into azide probe leads to a substantial increase in the sensitivity and reduced cell toxicity of CuAAC detection alkyne-tagged biomolecules. This will be of special value for the detection of low abundance targets or living system imaging. Cy5.5 Picolyl Azide is a bright and photostable near-IR probe that spectrally similar to Alexa Fluor® 680, DyLight® 680, and IRDye® 680 dye. The fluorescence of Cy5.5 dye is pH insensitive from pH 4 to pH 10 and produces minimal autofluorescence of biological specimens in this region of the spectrum. Fluorescence of this long-wavelength Cyanine dye is not visible to the human eye but is readily detected by most imaging systems.
- Molecular Weight:
- 119.26
- Shipping Conditions:
- Ambient
- Storage Conditions:
- -20[o]C desiccated. Avoid freeze/thaw cycles.
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Fluorophores
- Manufacturer's Data Sheet:Cy5.5-Picolyl-Azide
