CRISPR/Cas9 nuclease RGD-fiber modified Adenovirus (Ad(RGD)-Cas9)
Product Sizes
200 ul
£882.00
1902-200UL
About this Product
- SKU:
- 1902
- Additional Names:
- Ad(RGD)-Cas9|CRISPR/Cas9 nuclease RGD-fiber modified Adenovirus
- Buffer:
- DMEM, 2% BSA, 2.5% Glycerol
- Concentration:
- 1E10 PFU/ml
- Extra Details:
- Cas9 is the nuclease guided by the crRNA and tracrRNA (or trans-activating crRNA) to cleave specific DNA sequences. A guide RNA (gRNA) can be designed to include a hairpin that mimics the tracrRNA-crRNA complex. Binding specificity is based on the gRNA and a three nucleotide NGG sequence called the protospacer adjacent motif (PAM) sequence. With its ease in designing guide sequences to target specific genomic loci, the CRISPR/Cas system is a much simpler, faster, and robust alternative to TALEN and Zinc finger nuclease platforms. For site-specific genome editing, the CRISPR/Cas9 system requires the Cas 9 nuclease and the gRNA. This RGD-fiber modified adenovirus expresses a codon-optimized spCas9. It can be used along with guided RNA for genome-editing in cells that regular Ad5 doesn't infect well, including human or mouse macrophages, ES cells, T cells, synoviocytes, certain fibroblast and islet grafts etc., vectorbiolabs
- Shipping Conditions:
- Dry Ice
- Storage Conditions:
- -70[o]C Avoid freeze/thaw cycles.
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Viral Particles
- Manufacturer's Data Sheet:1902-crispr-cas9-nuclease-rgd-fiber-modified-adenovirus
