codon-optimized FLP Recombinase (FLPo) Adenovirus (Ad-CMV-FLPo)
Product Sizes
200 ul
£654.00
1775-200UL
About this Product
- SKU:
- 1775
- Additional Names:
- Ad-CMV-FLPo|codon-optimized FLP Recombinase (FLPo) Adenovirus
- Buffer:
- DMEM, 2% BSA, 2.5% Glycerol
- Concentration:
- 1E10 PFU/ml
- Extra Details:
- FLP from Saccharomyces cerevisiae, a site-specific recombinases (SSR) from the I>> integrase family, recognizes distinct 34 bp FRT sites. It has been widely used for generating conditional knockout mice, mediated by FLP/FRT system. Initial use of FLP in mammalian cells revealed inefficient recombinase activity due to thermo-instability of the FLP protein. Subsequent screening for thermo-stable mutants resulted in the identification of FLPe, with a 4-fold increase in recombination efficiency at 37C. However the recombination efficiency of FLPe in cells remains very low because of its non-mammalian origin. FLPo is a mouse codon optimized FLPe that greatly increases the FRT recombination efficiency for both in vivo and in vitro in mouse. This adenovirus express FLPo under a control of a CMV promoter., vectorbiolabs
- Shipping Conditions:
- Dry Ice
- Storage Conditions:
- -70[o]C Avoid freeze/thaw cycles.
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Viral Particles
- Manufacturer's Data Sheet:1775-codon-optimized-flp-recombinase-flpo-adenovirus
