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Lens Culinaris Agglutinin (LCA), Fluorescein

Product Sizes
5 mg
£185.00
FL-1041-5MG
About this Product
SKU:
FL-1041
Additional Names:
Lectins & Glycobiology Reagents [Lectins ], Lentil Lectin, Mannose binding Lectin
Application:
Immunofluorescence
Conjugate:
FITC
Extra Details:
LCA recognizes sequences containing I+/--linked mannose residues but recognizes additional sugars as part of the receptor structure, giving it a narrower specificity than Con A. An I+/--linked fucose residue attached to the N-acetylchitobiose portion of the core oligosaccharide significantly enhances affinity. By exploiting this narrower specificity, glycoproteins and glycopeptides can be subfractionated with LCA after initial isolation with Con A. Fluorescein labeled LCA has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.Lens culinaris agglutinin is composed of four subunits - two of about 17kDa and two of 8kDa. LCA recognizes sequences containing I+/--linked mannose residues but recognizes additional sugars as part of the receptor structure, giving it a narrower specificity than Con A. An I+/--linked fucose residue attached to the N-acetylchitobiose portion of the core oligosaccharide significantly enhances affinity. By exploiting this narrower specificity, glycoproteins and glycopeptides can be subfractionated with LCA after initial isolation with Con A.
Purification:
Affinity Purified
Shipping Conditions:
Ambient
Storage Conditions:
2-8[o]C
Supplier:
Vector Laboratories
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Lectins