Wisteria floribunda (WFA, Japanese Wisteria)
Product Sizes
5mg
£614.00
W1500-04D-5MG
About this Product
- SKU:
- W1500-04D
- Extra Details:
- Wisteria floribunda lectin is a 116,000 to 125,000 molecular weight glycoprotein with an isoelectric point of pH 5.4. Two subunits of about 60,000 daltons are produced in SDS gels, dissociating into 4 chains of about 30,000 daltons in the presence of reducing agents. The binding specificity is not completely clear but this lectin appears to preferentially bind carbohydrate structures terminating in N-acetylgalactosamine linked alpha or beta to the 3 or 6 position of galactose. Used to fractionate lymphocyte populations, the lectin although not mitogenic, elicits the production of lymphokines from murine splenocytes (references available upon request). Inhibiting/Eluting Sugar: 200mM N-acetylgalactosamine Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile 10mM HEPES buffered saline, pH 8.5, 0.1mM Ca++. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Molecular Weight:
- 116125
- Physical State:
- Supplied as a lyophilized powder. Reconstitute with 10mM HEPES buffered saline, pH 8.5, 0.1mM CaCl2. Do not vortex.
- Purity:
- Homogeneous by SDS-PAGE
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules:Lectins
