VAMP 1 (Vesicle Associated Membrane Protein 1, Vesicle-associated Membrane Protein 1, VAMP1, VAMP-1, Synaptobrevin 1, Synaptobrevin-1, SYB1, SYB-1)
Product Sizes
100ug
£812.00
V2024-01B-100UG
About this Product
- SKU:
- V2024-01B
- Application:
- Western Blot, Immunohistochemistry
- translate.label.attr.clone:
- 9L863
- Clonality:
- Monoclonal
- Extra Details:
- VAMP-1 (vesicle-associated membrane protein 1; also synaptobrevin-1/SYB1) is an 18kD member of the synaptobrevin family of proteins. It is expressed in neurons, neutrophils and skeletal muscle cells, and participates in vesicle fusion with the plasma membrane. Human VAMP-1 is a type IV transmembrane protein that contains an N-terminal cytoplasmic region (aa1-96) and a 22aa transmembrane domain (aa97-118). There is one coiled-coil region between aa33-93. Multiple splice variants are known that show two, three, four and 81aa substitutions for the C-terminal five amino acids. One three aa variant creates a mitochondrial targeting motif. over aa1-96, human VAMP-1 is 98% aa identical to mouse VAMP-1. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.2ug/ml, Daudi human Burkitt's lymphoma cell line, HepG2 human hepatocellular carcinoma cell line, L1.2 mouse pro-B cell line, and BaF3 mouse pro-B cell line Immunohistochemistry: 8-25ug/ml using immersion fixed paraffin-embedded sections of human brain (globus pallidus) Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Met1-Lys96 of human VAMP 1 expressed in E. coli.
- Isotype:
- IgG1
- Physical State:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes endogenous human VAMP-1 and VAMP-2 at ~16kD. Does not react with recombinant VAMP family members 5, 7, 8. Species Crossreactivity: mouse.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
