UMP-CMP Kinase, Recombinant, Human, aa1-228, His-Tag (CMPK1, CMPK, Deoxycytidylate Kinase, dCMP Kinase, UMP/CMPK, Uridine Monophosphate/Cytidine Monophosphate Kinase, Nucleoside-diphosphate Kinase)
Product Sizes
100ug
£576.00
U2281-05-100UG
About this Product
- SKU:
- U2281-05
- Extra Details:
- CMPK1, also known as UMP-CMP kinase and deoxycytidylate kinase, is an enzyme that catalyzes the phosphoryl transfer from ATP to UMP, CMP and dCMP. This enzymatic reaction leads to the formation of ADP and the corresponding nucleoside diphosphate, which are required for cellular nucleic acid synthesis. It also plays an important role in the activation of pyrimidine analogs, which are clinically useful anti-cancer and anti-viral drugs. Description: E. coli-expressed recombinant protein corresponding to aa1-228 of human CMPK1 (NCBI Accession # NP_057392) fused to His-tag at the N-terminus. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MLSRCRSGLL HVLGLSFLLQ TRRPILLCSP RLMKPLVVFV LGGPGAGKGT QCARIVEKYG YTHLSAGELL RDERKNPDSQ YGELIEKYIK EGKIVPVEIT ISLLKREMDQ TMAANAQKNK FLIDGFPRNQ DNLQGWNKTM DGKADVSFVL FFDCNNEICI ERCLERGKSS GRSDDNRESL EKRIQTYLQS TKPIIDLYEE MGKVKKIDAS KSVDEVFDEV VQIFDKEG Molecular Weight: ~28kD (248aa), confirmed by MALDI-TOF Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Molecular Weight:
- 28
- Physical State:
- Supplied as a liquid in 20mM Tris-HCl buffer, pH 8, 100M sodium chloride, 1mM DTT, 20% glycerol.
- Purity:
- ~90% (SDS-PAGE)
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Molecular Biology Reagents:Nucleotides
