Urate Transporter 1 (URAT1) (Not for Export EU)
Product Sizes
100ul
£704.00
U2005-01-100UL
About this Product
- SKU:
- U2005-01
- Clonality:
- Polyclonal
- Extra Details:
- Urate, a naturally occurring product of purine metabolism, is a scavenger of oxidants. Urate exists primarily as a weak acid (pKa ~5.75). Urate concentration is quite high (~200-500 uM) in human due to loss of uricase gene and the presence of an effective reabsorption system. Recently an OAT-related transporter, URAT1, has been identified in the kidney that acts as urate-anion exchanger and effectively regulates urate levels. URAT1/RST/SLC22A12 (mouse 553-aa, human 555-aa, chromosome 11q13, ~60 kD) is ~42% identical with OAT4. Like OAT4, it is predicted to display 12-TM domains with cytoplasmic N and C-terminus. URTA1 is prominent is epithelial cells of proximal tubule of the renal cortex. Patients with idiopathic renal hypouricemia (lack of blood uric acid) have defects in URAT1 gene. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 using ECL technique ELISA: 1:10,000-50,000. Coat ELISA plate with 1ug/ml control peptide. Optimal dilutions to be determined by the researcher. Control Peptide: U2005 Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Rabbit
- Immunogen:
- Synthetic 11-aa peptide sequence near the cytoplasmic, C-terminus of human URAT1 ( KLH coupled).
- Isotype:
- IgG
- Physical State:
- Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
- Purity:
- Serum
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes Urate Transporter 1 (URAT1). Peptide Sequence Homology: human URAT1/RST or OAT-4-like protein: 100%; mouse RST/URAT1: 54%.; no significant homology with other OAT4 or other proteins.
- Source:
- human
- Storage Conditions:
- 4[o]C (-20[o]C Glycerol)
- Type:
- Antibodies:Polyclonal Antibody
