UbcH1, E2-25K (Huntingtin Interacting Protein 2, HIP2)
Product Sizes
50ug
£448.00
U0850-03B-50UG
About this Product
- SKU:
- U0850-03B
- Application:
- Western Blot
- translate.label.attr.clone:
- 8C346
- Clonality:
- Monoclonal
- Extra Details:
- Protein ubiquitinylation requires the concerted action of the E1, E2 and E3 ubiquitin-conjugating enzymes. Ubiquitin is first activated through an ATP-dependent formation of a thiol ester with E1. The activated ubiquitin is then transferred to a thiol-group of ubiquitin-conjugation enzyme E2. The final step is the transfer of ubiquitin from E2 to an e-amino group of the target protein lysine residue, which is mediated by ubiuitin-conjugation enzyme E3. E2-25K (Hip2) is a member of the E2 protein family that catalyzes multiubiquitin chain synthesis via Lys48 of ubiquitin. E2-25K is reportedly involved in Alzheimer's disease, Huntington's disease and antigen processing through its interaction with amyloid-b, huntingtin, and MHC-heavy chain proteins. Lys14 of E2-25K can be modified by SUMOylation, with this modification resulting in inhibited E2 activity. Applications: Suitable for use in Dot Blot and Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- Partial sequence of recombinant full-length human UbcH1, E2-25K
- Isotype:
- IgG1
- Physical State:
- Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human UbcH1, E2-25K.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
