Tumor Necrosis Factor alpha (TNFa)
Product Sizes
100ug
£576.00
T9160-30A-100UG
About this Product
- SKU:
- T9160-30A
- Application:
- Western Blot
- Clonality:
- Polyclonal
- Extra Details:
- Tumor Necrosis Factor alpha (TNF-alpha) is a 17.3kD multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts. TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumor cell lines and the stimulation of cell differentiation and proliferation under certain conditions. Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis. Applications: Suitable for use in Neutralization, ELISA and Western Blot. Other applications have not been tested. Recommended Dilution: Neutralization: 0.07-0.1ug/ml will yield one-half maximal inhibition [ND50] of the biological activity (2.0ng/ml). ELISA: 0.5-2ug/ml is detection by direct ELISA (using 100ul/well antibody solution). In conjunction with compatible secondary reagents this antibody allows detection of 0.2-0.4ng/well of recombinant rat TNF-a. Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Country of Origin: USA
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to rat Tumor Necrosis Factor alpha expressed in E. coli.
- Isotype:
- IgG
- Physical State:
- Supplied as a lyophilized powder from PBS, pH 7.4. Reconstitute with 100ul sterile dH2O.
- Purity:
- Purified by Immunoaffinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes rat Tumor Necrosis Factor alpha (TNFa).
- Source:
- rat
- Storage Conditions:
- -20[o]C
- Type:
- Antibodies:Polyclonal Antibody
