Tumor Necrosis Factor alpha (TNFa) (HRP)
Product Sizes
100ul
£812.00
T9160-30A-HRP-100UL
About this Product
- SKU:
- T9160-30A-HRP
- Application:
- Western Blot
- Clonality:
- Polyclonal
- Extra Details:
- Tumor Necrosis Factor alpha (TNF-alpha) is a 17.3kD multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts. TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumor cell lines and the stimulation of cell differentiation and proliferation under certain conditions. Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis. Applications: Suitable for use in Neutralization, ELISA and Western Blot. Other applications have not been tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to rat Tumor Necrosis Factor alpha expressed in E. coli
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish peroxidase (HRP)
- Purity:
- Purified by Immunoaffinity chromatography
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes rat Tumor Necrosis Factor alpha (TNFa)
- Source:
- rat
- Storage Conditions:
- -20[o]C
- Type:
- Antibodies:Polyclonal Antibody
