Tumor Necrosis Factor alpha (TNFa) (HRP)
Product Sizes
100ul
£1088.00
T9160-18A-HRP-100UL
About this Product
- SKU:
- T9160-18A-HRP
- Application:
- Immunohistochemistry
- translate.label.attr.clone:
- 5E852
- Clonality:
- Monoclonal
- Extra Details:
- Tumor Necrosis Factor alpha (TNF-alpha) is a 17.3kD multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts. TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumor cell lines and the stimulation of cell differentiation and proliferation under certain conditions. Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis. Applications: Suitable for use in Immunohistochemistry, ELISA and Inhibition of biological activity of TNFa in different biological fluids. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Formalin-fixed frozen tissues Optimal dilutions to be determined by the researcher. Hybridoma: X63.Ag8.653 myeloma cells with spleen cells of Balb/c mice. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human TNF-alpha.
- Isotype:
- IgG1
- Physical State:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with horseradish peroxidase (HRP).
- Purity:
- Purified by Protein A affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human TNFa. Effective in the inhibiting the biological activity of recombinant and naturtal TNFa.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Type:
- Antibodies:Monoclonal Antibody
