TSC (Thiazide-sensitive Na Cl Transporter, NCC) (Not for Export EU)
Product Sizes
100ul
£704.00
T9101-10-100UL
About this Product
- SKU:
- T9101-10
- Clonality:
- Polyclonal
- Extra Details:
- Chloride is a critical component of all living cells. It is also the single most dominant diffusible anion inside of most cells-the others are mostly impermeable organic anions. Three groups of proteins have been identified in CCC family based upon their structures, ligands, and inhibitors. These are the thiazide-sensitive Na+-Cl-cotransporters (NCC or TSC), the loop diuretics-sensitive Na+-K+-Cl- (NKCC) cotransporters (NKCC1/CCC1/BSC2 and NKCC2/CCC2/BSC1), and the K+-Cl--cotransporters (KCC1-4). These co-transporters share a common predicted membrane topology, with 12 TM domains (~500 aa), and long hydrophilic, intracellular N-and C-termini containing regulatory phosphorylation sites. NKCC transport Na, K, and Cl ions into and out of a wide variety of epithelial and non-epithelial cells. The transport process is characterized by electroneutrality (almost always with stoichiometry of 1Na:1K:2Cl) and inhibition by the loop diuretic bumetanide, benzametanide, and furosemide. In the distal convoluted tubule (DCT) of the kidney, the primary apical entry pathway for Na is via the thiazide-sensitive Na-Cl transporter (TSC or NCC; human 1021 aa, rat/mouse 1002 aa; ~60 % identity with NKCC). TSC is specifically detected at the apical cell membrane of DCT. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 using ECL. ELISA: 1:10,000-50,000. T9100-12 Control peptide can be used to coat ELISA plates at 1ug/ml. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to an 18aa sequence near the N-terminus of rat TSC.
- Isotype:
- IgG
- Physical State:
- Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
- Purity:
- Serum
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes rat Thiazide-Sensitive Na-Cl Transporter (TSC, NCC). The immunogen shows no significant sequence homology with other CCC. Species sequence homology: mouse and rabbit: 98% and human 83%.
- Source:
- rat
- Storage Conditions:
- 4[o]C (-20[o]C Glycerol)
- Type:
- Antibodies:Polyclonal Antibody
