Troponin I, Cardiac, aa169-184 (cTnI)
Product Sizes
1mg
£991.00
T8665-16S-1MG
About this Product
- SKU:
- T8665-16S
- Application:
- Western Blot
- translate.label.attr.clone:
- 7E149
- Clonality:
- Monoclonal
- Extra Details:
- Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits, the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI). Troponin I binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place, because of which myosin cannot bind to actin in relaxed muscle. When Calcium binds to the Troponin C it causes certain confomational changes which leads to dislocation of troponin I. The letter “I” is given due to its inhibitory character. The tissue specific subtypes are: Slow-twitch skeletal muscle isoform troponin I, TNNI1 (1q31.3, 191042) Fast-twitch skeletal muscle isoform troponin I, TNNI2 (11p15.5, 191043) Cardiac troponin I, TNNI3 (19q13.4, 191044) Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Mouse
- Immunogen:
- Free human cardiac troponin
- Isotype:
- IgG2a
- Physical State:
- Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
- Purity:
- Purified by Protein A affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Reacts equally with free cardiac troponin I (cTnl) and cTnl forming complexes with other troponin components. Not affected by heparin, phosphorylation and oxidation. Recognizes an epitope located between aa169-184 of cTnI. Less than 10% crossreactivity with skeletal muscle troponin I.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
