TRIM (T Cell Receptor-Interacting Molecule)
Product Sizes
100ug
£800.00
T8464-35C-100UG
About this Product
- SKU:
- T8464-35C
- Application:
- Flow Cytometry, Western Blot
- translate.label.attr.clone:
- 9H187
- Clonality:
- Monoclonal
- Extra Details:
- Human TRIM (T cell receptor-interacting molecule) (also trat1, T cell receptor-associated transmembrane adaptor 1, and pp29/30) is a 30kD, type III transmembrane protein, that is a member of the transmembrane adaptor protein (TRAP) family. It contains a short, 8aa extracellular region, a 19aa transmembrane region, and a 159aa cytosolic tail. Its cytoplasmic tail contains several tyrosine motifs with the potential to bind to Src-homology 2 (SH2) domains of signaling proteins. TRIM is present in T cells and NK cells. Human TRIM shares 66% aa sequence identity with mouse TRIM. Applications: Suitable for use in Flow Cytometry, ELISA, and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 25ug/ml ELISA: 0.5-1.0ug/ml Western Blot: 1-2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Mouse
- Immunogen:
- Recombinant human TRIM, aa50-186; Q6PIZ9 (E. coli).
- Isotype:
- IgG1
- Physical State:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Purity:
- Purified by Protein G affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human TRIM.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
