TRAIL (TNF-Related Apoptosis Inducing Ligand, Apo2 Ligand)
Product Sizes
500ug
£435.00
T8180-04-500UG
About this Product
- SKU:
- T8180-04
- Application:
- Western Blot, Apoptosis
- translate.label.attr.clone:
- 4i170
- Clonality:
- Monoclonal
- Extra Details:
- Applications: Suitable for use in ELISA, Western Blot and Neutralization. Other applications have not been tested. Recommended Dilutions: ELISA (Sandwich): 2-4ug/ml (100ul/well) detects at least 500pg/ml of recombinant human TRAIL when used with biotinylated anti-human TRAIL (catalog# 143686) as detection antibody at a concentration of 1-2ug/ml. Western Blot: 0.25-0.5ug/ml. The detection limit is 0.25-0.5ng/lane of recombinant human TRAIL, under either reducing or non-reducing conditions. Neutralization: 0.3-0.6ug/ml to yield one-half the maximal inhibition of the biological activity of human TRAIL (10ng/ml). Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human TRAIL derived from E. coli.
- Isotype:
- IgG1,k
- Physical State:
- Supplied as a lyophilized powder from PBS. Reconstitute with 500ul of sterile ddH2O.
- Purity:
- Purified by Protein A affinity chromatography from ascites.
- Shipping Conditions:
- Ambient
- Specificity:
- Recognizes human TRAIL.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
