Themis (Protein THEMIS, Grb2-associating Protein, Gasp, Protein Thylex, Thymocyte-expressed Molecule Involved in Selection)
Product Sizes
100ug
£767.00
T3773-21-100UG
About this Product
- SKU:
- T3773-21
- Application:
- Flow Cytometry, Western Blot
- translate.label.attr.clone:
- 12K186
- Clonality:
- Monoclonal
- Extra Details:
- Themis also called Gasp (Grb2-associating protein), is a 72kD, 641aa cytosolic phosphoprotein mainly expressed in late double-negative and double-positive thymocytes. It is involved in thymocyte positive and negative selection through regulation of TCR signaling. Within the region used as an immunogen, mouse Themis shares 87% and 96% aa sequence identity with human and rat Themis. Applications: Suitable for use in Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 2.5ug labels 10e6 Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to Lys122-Lys237 from mouse Themis expressed in E.coli.
- Isotype:
- IgG2b
- Physical State:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Purity:
- Purified by Protein G affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes mouse Themis. Species sequence homology: Recombinant human Themis aa2-282 (100%).
- Source:
- mouse
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
