Telomerase (EST2, hEST2, TCS1, Telomerase Catalytic Subunit, Telomerase-associated protein 2, Telomere Reverse Transcriptase, TERT, TP2, TRT) (Not for Export EU)
Product Sizes
100ul
£704.00
T2399-85-100UL
About this Product
- SKU:
- T2399-85
- Clonality:
- Polyclonal
- Extra Details:
- The 3'ends of chromosomes are capped with telomere sequences (TTAGGG; 6-26 nucleotides in length) by ribonucleoprotein telomerase during DNA replication. Telomerase is an unusual RNA-dependent DNA polymerase that uses and RNA component to specify the addition of telomere. The telomeric RNA contains a sequence complementary to TTAGGG. In ciliated protozoa and yeast, telomere length is maintained by regulating the activity of telomerase. Many mammalian cells do not express telomerase resulting into shortening of telomere with each cell division, and ultimately causing the chromosomal instability, aging and cell death. Approx. 4.8 kb of telomeric DNA is lost with each cell division resulting into large number of chromosomal abnormalities. Purification of telomerase from the ciliate also revealed two protein of 43 and 123kD. p123 is a homolog of yeast Est2 (Essential for Telomerase activity) and show 20% homology. Mammalian homologs of yeast Est2 (also known as TP2 for Telomerase associated Protein 2; hEST2 or telomerase catalytic subunit or telomerase reverse transcriptase, TERT) have also been cloned. Est2 is associated with telomerase activity and TP1. Mouse/rat and human TP1 are 2627 and 2629 aa proteins, respectively. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilutions: ELISA: 1:10,000-50,000 for neat serum. Control peptide (Catalog #T2399-80) can be used to coat ELISA plates at 1ug/ml. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to 23aa near the N-Terminus of yeast Telomerase, conjugated to KLH
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in PBS, 0.09% sodium azide
- Purity:
- Serum
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes Telomerase. No significant sequence homology with mammalian telomerases
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
