TCR, alpha, beta (T Cell Receptor, T-cell Receptor beta-1 Chain C Region, TRBC1, T-cell Receptor alpha Chain C Region, TRAC, TCRA) (PE/CY5)
Product Sizes
100ug
£384.00
T2040-02N5-100UG
About this Product
- SKU:
- T2040-02N5
- Application:
- Immunoprecipitation, Flow Cytometry, Immunohistochemistry
- translate.label.attr.clone:
- H57-597
- Clonality:
- Monoclonal
- Extra Details:
- The alpha/beta TCR is expressed on T lymphocytes of all mouse strains tested.2 Plate-bound or soluble antibody activates a/b TCR-bearing T cells.2 In vitro and in vivo, this antibody can induce immature thymocytes to undergo apoptosis.3,4 In vivo administration of this antibody has been shown to deplete a/b TCR-bearing cells to near completion and prevent graft rejection.5 Applications: • Flow cytometry 2,6,7 • Immunohistochemistry (acetone-fixed, frozen sections and formalin-fixed, paraffin-embedded sections) • Immunoprecipitation 2,8 • In vitro stimulation of a/b TCR-expressing T cells 2 • In vitro and in vivo depletion of a/b TCR-bearing cells 9 Recommended Dilution: Flow Cytometry: ≤ 0.2ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. PE/CY5 combination conjugate is used as a third fluorochrome in multicolor flow cytometry applications when only a single 488nm excitation source is available. Antibodies are covalently coupled using heterobifunctional linking reagents, resulting in an optimal conjugation format for flow cytometry.
- Host:
- Hamster
- Immunogen:
- TCR affinity purified from mouse T-cell hybridoma DO-11.10
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with PE/CY5.
- Purity:
- Purified
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes common epitope of the beta chain of the mouse alpha/beta T cell receptor (TCR) complex. Does not react with gamma, delta TCR-bearing cells.
- Source:
- mouse
- Storage Conditions:
- 4[o]C Do Not Freeze
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
