TCCR (T Cell Cytokine Receptor, IL-27 R alpha, IL27RA, WSX-1)
Product Sizes
500ug
£1042.00
T2009-04B1-500UG
About this Product
- SKU:
- T2009-04B1
- Application:
- Western Blot
- translate.label.attr.clone:
- 11n183
- Clonality:
- Monoclonal
- Extra Details:
- TCCR, also known as WSX-1 and IL-27 Ra, is a 96kD member of the type I group 2 cytokine receptor family.1-4 The extracellular region of human TCCR shares 63% aa identity with the extracellular domain of mouse IL-27 Ra.2, 3 TCCR is expressed in mast cells, endothelial cells, NK cells, macrophages, monocytes, B cells, dendritic cells, and native T cells.1, 2, 4, 5 As with many other class I cytokine receptor chains, IL-27 Ra heterodimerizes with gp130, the common receptor subunit of IL-6 family cytokines, to form a functional IL-27 receptor.6 IL-27 only binds to IL-27 Ra/WSX-1/TCCR and not the gp130 subunit.7 Studies using WSX-1 mice reveal that WSX-1 has the ability to suppress T cell hyperactivity during infection, and to mediate an inhibition of both type 1 and type 2 T cell immunity.4, 8, 9 In particular, WSX-1 is believed to induce IL-12 R on native T cells, predisposing them to a Th1 phenotype. This is offset by its ability to limit IL-12 (and TNF) production by macrophages, and its suppression of IFN-g production. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Hybridoma: NSO myeloma cells from Balb/c mice. Absorption: 488nm Emission: 565-605nm Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human TCCR expressed in NSO cells (Q6UWB1).
- Isotype:
- IgG2b
- Physical State:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
- Purity:
- Purified by Protein A affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human TCCR. No crossreactivity with recombinant human gp130 or recombinant mouse IL27 Ra was observed by direct ELISA using the unconjugated form of this antibody.
- Source:
- human
- Storage Conditions:
- 4[o]C Do Not Freeze
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
