TARC, Human (Thymus and Activation Regulated Chemokine)
Product Sizes
100ug
£576.00
T1020-05-100UG
About this Product
- SKU:
- T1020-05
- Application:
- Western Blot
- Clonality:
- Polyclonal
- Extra Details:
- Human TARC is a recently discovered protein belonging to the beta-chemokine (C-C) family of cytokines, secreted by activated monocytes, thymic and lymph node dendritic cells. It is chemotactic towards activated T cells (Th2 subset) which express express CCR4 but ineffective on monocytes. Human TARC is an 8.0kD protein containing 71 amino acid residues. Applications: Suitable for use in Neutralization, ELISA and Western Blot. Other applications not tested. Recommended Dilution: Neutralization: 2-3ug/ml will yield one-half maximal inhibition [ND50] of the biological activity of human TARC (100ng/ml). ELISA (Sandwich): 0.5-2ug/ml using 100ul/well antibody solution In conjunction with biotylated anti-human TARC (T1020-05A) as a detection antibody, allows the detection of at least 2000-4000pg/well of recombinant human IL-22. Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human TARC is 1.5-3ng/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to human TARC expressed in E. coli.
- Isotype:
- IgG
- Physical State:
- Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
- Purity:
- Purified by immunoaffinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human TARC.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
