STAT 1, phosphorylated (Tyr701) (AlexaFluor® 488)
Product Sizes
100ul
£748.00
S7969-10A-100UL
About this Product
- SKU:
- S7969-10A
- Application:
- Flow Cytometry
- translate.label.attr.clone:
- 7H64(58D6)
- Clonality:
- Monoclonal
- Extra Details:
- Stat1, while activated in response to a large number of ligands, appears to be essential for responsiveness to IFN-alpha and IFN-gamma. Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation and DNA binding. Stat1 has two isoforms, Stat1apha (91kD) and the splice variant Stat1beta (84kD). In most cells, both isoforms are activated by IFN-alpha, but only Stat1alpha is activated by IFN-gamma. Stat1 has been found to be inappropriately activated in many tumors. In addition to tyrosine phosphorylation, Stat1 is phosphorylated through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway at Ser727 in response to IFN-alpha and other cellular stresses. Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: (Fixed/Permeabilized): 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt at 4°C. Freezing Alexa Fluor® conjugates will result in a substantial loss of activity. Alexa Fluor® conjugates are sensitive to light. Note: We do not recommend adding glycerol as a cryopreservative.
- Host:
- Rabbit
- Immunogen:
- Synthetic phospho peptide corresponding to residues surrounding Tyr701 of human Stat1.
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in PBS, pH 7.2, 2mg/ml BSA, 0.09% sodium azide. Labeled with Alexa Fluor® 488. Fluorophore/Protein ratio: 2-6.
- Purity:
- Purified prior to conjugation
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes endogenous levels of human phospho(Tyr701) Stat1. Also recognizes p91 Phospho(Tyr701) Stat1 and p84 Phospho-Stat1 splice variants. Species Crossreactivity: mouse.
- Source:
- human
- Storage Conditions:
- 4[o]C Do Not Freeze
- Type:
- Antibodies:Monoclonal Antibody
