Staphylococcus aureus Enterotoxin B (SEB)
Product Sizes
100ug
£214.00
S7965-35B-100UG
About this Product
- SKU:
- S7965-35B
- Application:
- Western Blot
- translate.label.attr.clone:
- 3G314
- Clonality:
- Monoclonal
- Extra Details:
- Staphylococcal enterotoxins represent a group of proteins, which are secreted by Staphylococcus aureus and cause the intoxication staphylococcal food poisoning syndrome. The illness characterised by high fever, hypotension, diarrhea, shock, and in some cases death. Their molecular masses range between 27 and 30kD. At present, seven enterotoxins are known, namely A, B, C1, C2, C3, D and E. Their aa sequences have been determined and it was shown that all are single chain polypeptides containing one disulfide bond formed by two half cystines located in the middle of the polypeptide chain, which form the so called cysteine loop. Enterotoxins are extremely potent activator of T cells, stimulating the production and secretion of various cytokines which mediate many of the toxic effects of these substances. Enterotoxins are Superantigens, inducing polyclonal T cell activation by binding to the TCR and to the alpha chain of the MHC II molecule simultaneously. Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Hybridoma: X63-Ag8-653 myeloma cells with spleen cells from Balb/c mice. Recommended Pair: Suitable for use as the capture or solid phase antibody. Matched to S7965-35A as the detection or conjugate (label) antibody. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- Recombinant S. aureus enterotoxin B expressed in E. coli.
- Isotype:
- IgG1
- Physical State:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide
- Purity:
- Purified by Protein G affinity chromatography from ascites. ≥95% (SDS PAGE).
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes Staphylococcus aureus Enterotoxin B (SEB). Does not crossreact with S. aureus enterotoxins A, C, D and E.
- Source:
- S.aureus
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
