Stage Specific Embryonic Antigen 4 (SSEA4) (APC)
Product Sizes
100Tests
£1010.00
S6610-11A-100TESTS
About this Product
- SKU:
- S6610-11A
- Application:
- Flow Cytometry
- translate.label.attr.clone:
- 8H218
- Clonality:
- Monoclonal
- Extra Details:
- Stage-Specific Embryonic Antigens (SSEAs) were originally identified by monoclonal antibodies (mAb) generated against defined carbohydrate epitopes associated with lacto- and globo-series glycolipids (1). The SSEA-4 specific monoclonal antibody was generated from a mouse immunized with the 2102Ep human teratocarcinoma cell line (1). This mAb recognizes the terminal structure of the sialyl galactosylgloboside Luke (LKE) antigen expressed on human red blood cells and platelets (2, 3). SSEA-4 expression has been reported on ACHN renal tubular cells (4) and also on normal human kidney (5). The expression of SSEA-4 on human teratocarcinoma-derived cell lines (1, 6) prompted investigations into the expression of globosides during embryonal differentiation. Embryonic carcinoma cells that express SSEA-4 rapidly lose expression of SSEA-4 as the cells differentiate (7, 8). In addition, human embryonic stem cells express SSEA-4 (9). Studies have utilized this mAb for the detection of SSEA-4 on mouse oocytes through the early blastocyst stage (1). However, SSEA-4 has not been detected on mouse embryonic carcinoma or mouse embryonic stem cells (1). Finally, studies have shown that SSEA-4 is expressed on primary sensory neurons of the rat and other species (10). These investigations imply a role for SSEA-4 and other cell surface carbohydrates in cellular adhesion (10). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul labels 1x10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- 2120Ep human embryonal carcinoma cell line
- Isotype:
- IgG3
- Physical State:
- Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human SSEA-4. Species Crossreactivity: mouse
- Source:
- human
- Storage Conditions:
- 4[o]C Do Not Freeze
- Type:
- Antibodies:Monoclonal Antibody
